Process for extracting lupinol from betulin category plant

A technology of lupeol and betula, applied in the field of extracting lupeol, which can solve the problem of easy cross-diffusion of components

Inactive Publication Date: 2006-06-28
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is difficult to use in industrial production, because usually the components of plant extracts are too complex, and many components are easy to cross-diffusion when the extract is directly prepared by chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: according to the technical scheme of the present invention, extract the lupeol in the Betula plant and carry out according to the following steps:

[0017] (1) extraction

[0018] Take 200 grams of dried red birch bark and crush it into a 20-mesh coarse powder, add 2L of dichloromethane to reflux extraction, the first two times for two hours, and the third time for 1 hour, combine the extracts, concentrate them into small bodies, and place a small amount of The gray-brown precipitate was filtered again, and the filtrate was concentrated to obtain a crude product, which was further washed with a small amount of cyclohexane several times to remove low-polarity impurities to obtain a pale yellow crude product.

[0019] (2) Purification

[0020] The above crude product was dissolved by heating under reflux with a mixed solution of absolute ethanol and acetone, left to crystallize, and recrystallized twice in the same way to obtain white needle crystals (conte...

Embodiment 2

[0023] Embodiment 2: The difference between this embodiment and embodiment 1 is that the birch plant used in step 1 is maple birch bark.

Embodiment 3

[0024] Embodiment 3: The difference between this embodiment and embodiment 1 is that in step 1, chloroform is used for reflux extraction.

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PUM

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Abstract

The invention discloses a method to distill lupeol from birch genus plant that includes the following steps: smashing the dry birch bark, using methylene chloride to take back flowing and distilling, recycling methylene chloride, placing for deposition, filtering, removing filtrate and the washing deposit by a little cyclohexane several time to remove the low polarity impurity; recrystallizing for three times by absolute ethyl alcohol and acetone, the lupeol would be gained. The invention has the advantage of low cost, common raw material, simple technology.

Description

[0001] Field [0002] The invention belongs to the research of natural products, relates to the extraction of natural products with important application value, in particular to a method for extracting lupeol from birch plants. Background technique [0003] Lupeol has obvious antihypertensive and anti-inflammatory effects. Nikinema et al. conducted anti-croton oil-induced ear edema experiments on lupeol and its semi-synthetic organic acid ester derivatives. The results showed that at 0.4 μmol / L per ear At the same dose, they all have inhibitory activity, among which the hemisuccinate of lupeol has the strongest activity and its inhibition rate is 90%, while at the same dose, the inhibition rate of the anti-inflammatory drug indomethacin is 73%. [0004] Lupeol also has the effect of inhibiting tumor growth. Studies have shown that lupeol can increase the number of hypodiploids in apoptotic cells of human leukemia cells by 28.5% at a concentration of 75 μmol / L, and it is concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07J75/00
Inventor 方玉春曹秀萍严孝金刘培培顾谦群朱伟明王长云
Owner OCEAN UNIV OF CHINA
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