Fungus possessing eelworm-killing activity and its preparation method and uses
A technique for killing nematodes and fungi, which is applied in the field of fungi for poisoning and killing plant parasitic nematodes and its preparation, which can solve the problems of large waste of chemical nematicides, environmental pollution, and damage to soil biota
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Embodiment 1
[0019] Example 1: Cultivation of strain Beauveria bassiana snf0907
[0020] The mycelium of B. bassiana snf0907 was inoculated on the test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; water 1000mL. Incubate at 25°C for 10 days to obtain test tube species.
[0021] Inoculate the test tube into 250mL Erlenmeyer flask (50mL per bottle) liquid medium again, the medium formula is (percentage by weight): sucrose (2.83%), ammonium sulfate (1.367%), K 2 HPO 4 (0.064%), MgSO 4 ·7H 2 O (0.182%), KCl (0.996%), FeSO 4 (0.02%), the rest is water, pH 6.5. Inoculate the test tube seed into a 250mL Erlenmeyer flask (50mL per bottle) liquid medium, at 25°C-28°C, shaker at 150r min -1 ~200r·min -1 , Fermentation 120h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0022] Example 2: Cultivation of strain Beauveria bassiana snf0907
[0023] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:
[0024] Liquid medium formula is: sucrose (3%), ammonium sulfate (5%), K 2 HPO 4 (0.08%), MgSO 4 ·7H 2 O(0.4%), KCl(2.0%), FeSO 4 (0.002%), pH7.
Embodiment 3
[0025] Example 3: Cultivation of strain Beauveria bassiana snf0907
[0026] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:
[0027] Liquid medium formula is: sucrose (6%), ammonium sulfate (3%), K 2 HPO 4 (2%), MgSO 4 ·7H 2 O(0.5%), KCl(5.2%), FeSO 4 (0.04%), pH6.
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