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Fungus possessing eelworm-killing activity and its preparation method and uses

A technique for killing nematodes and fungi, which is applied in the field of fungi for poisoning and killing plant parasitic nematodes and its preparation, which can solve the problems of large waste of chemical nematicides, environmental pollution, and damage to soil biota

Inactive Publication Date: 2006-07-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has poor selectivity, strong extinction, and damages soil biota. Zhang Keqin, He Shichuan, Zhou Wei, etc. 1991. The role and research progress of fungi and bacteria in nematode biocontrol. Insecticidal Microbes. 3: 55-66), the 21st century is called the environmental protection century, some effective chemical nematicides Therefore, the research on the biological control of plant parasitic nematodes has naturally become a hot issue.

Method used

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  • Fungus possessing eelworm-killing activity and its preparation method and uses
  • Fungus possessing eelworm-killing activity and its preparation method and uses
  • Fungus possessing eelworm-killing activity and its preparation method and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Cultivation of strain Beauveria bassiana snf0907

[0020] The mycelium of B. bassiana snf0907 was inoculated on the test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; water 1000mL. Incubate at 25°C for 10 days to obtain test tube species.

[0021] Inoculate the test tube into 250mL Erlenmeyer flask (50mL per bottle) liquid medium again, the medium formula is (percentage by weight): sucrose (2.83%), ammonium sulfate (1.367%), K 2 HPO 4 (0.064%), MgSO 4 ·7H 2 O (0.182%), KCl (0.996%), FeSO 4 (0.02%), the rest is water, pH 6.5. Inoculate the test tube seed into a 250mL Erlenmeyer flask (50mL per bottle) liquid medium, at 25°C-28°C, shaker at 150r min -1 ~200r·min -1 , Fermentation 120h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.

Embodiment 2

[0022] Example 2: Cultivation of strain Beauveria bassiana snf0907

[0023] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0024] Liquid medium formula is: sucrose (3%), ammonium sulfate (5%), K 2 HPO 4 (0.08%), MgSO 4 ·7H 2 O(0.4%), KCl(2.0%), FeSO 4 (0.002%), pH7.

Embodiment 3

[0025] Example 3: Cultivation of strain Beauveria bassiana snf0907

[0026] Basically the same as embodiment one, the difference is the liquid culture medium formula, and its formula is:

[0027] Liquid medium formula is: sucrose (6%), ammonium sulfate (3%), K 2 HPO 4 (2%), MgSO 4 ·7H 2 O(0.5%), KCl(5.2%), FeSO 4 (0.04%), pH6.

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Abstract

The invention relates to a fungus metabolism product with destroying intectinal worm's effect and it's preparing method and application in the field of microorganism pesticides technique. The preserved number of the fungus bacterial strain: Beauveria bassiana snf0907 is CGMCC No.1340.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04
Inventor 段玉玺刘霆陈立杰王媛媛李永峰薛春生
Owner SHENYANG AGRI UNIV
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