Method for detecting gymnosperm pollen in-tube antigen

Abstract

The present invention discloses a method for detecting gymnosperm pollen tube interior antigen. Said method includes the following steps: (1), using phosphoric acid buffer solution containing paraformaldehyde to fix pollen tube, using zymolysis solution containing cellulose and macerozyme to zymolyze gymnosperm pollen tube, using Triton X-100 or NP-40 aqueous solution whose mass percentage concentration is 0.2-1% to wash the pollen tube; and (2), making the pollen tube treated by step 1 and the correspondent antibody for resisting the antigen to be tested undergo the process of immunofluorescence reaction, if the fluorescence is detected, the gymnosperm pollen tube interior contains antigen to be tested and can obtain the location condition of said antigen to be tested.

Description

A method for detecting antigens in gymnosperm pollen tubes technical field The invention relates to a method for detecting antigens in gymnosperm pollen tubes, in particular to a method for detecting antigens in gymnosperm pollen tubes by means of fluorescence immunity technology. Background technique As the carrier of the male reproductive unit in the fertilization process of flowering plants, the pollen tube carries and transmits the sperm cells to the ovule, realizes the combination of sperm and eggs, and completes fertilization. Pollen tube is a typical haploid cell with apical growth characteristics and independent existence. It shows a uniform columnar polar growth pattern when grown in vitro, so it is considered to be an ideal cell system for studying polar growth in the field of biotechnology. In addition, as a model system, pollen tube is also of great significance for in-depth study of cell polar growth, cell-cell interaction and signal transduction. Since the p...

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Problems solved by technology

In addition, for some protein antigens, due to the easy inactivation of the antigen during the fixation process and the limitation of the time and temperature conditions required for the antigen-antibody reaction process, it is difficult to detect and locate the antigen in the pollen tube, and there is no related gymnosperm so far. A proven method for the detection and localization of antigens in pollen tubes has led to a lag in biological research related to gymnosperms relative to angiosperms

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