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Plant worms mycelium extracat fraction and composition for oral intake

A technology of Cordyceps sinensis mycelium and mycelium, which is applied in the fields of food and drink compositions, external medicines, oral ingestion compositions, food and beverages, medicines for preventing or treating cancer, and pharmaceutical compositions, and can solve the problem of active ingredient content And it is hard to say that the activity is very sufficient, etc.

Active Publication Date: 2006-08-23
KOBAYASHI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Attention has been paid to such physiological activities, and attempts have been made to effectively ingest active ingredients (see Patent Document 1), but it is difficult to say that the content and activity of the active ingredients in the extract are sufficiently sufficient.

Method used

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  • Plant worms mycelium extracat fraction and composition for oral intake
  • Plant worms mycelium extracat fraction and composition for oral intake
  • Plant worms mycelium extracat fraction and composition for oral intake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] [Example 1] Preparation of the isolate of Cordyceps sinensis mycelium

[0046] In the implementation of the present invention, the powder of the Cordyceps sinensis mycelium culture that becomes the raw material can be used, for example, "fermented Cordyceps fungus powder" commercially available in China (trade name: CORBRIN CAPSULE, manufacturing company: Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd.) .

[0047] The raw material Cordyceps sinensis mycelium powder is extracted with 99.5% ethanol at 90-95° C. for 6 hours. The amount of ethanol is 2 mL per 1 g of mycelia. The residue is filtered, and the residue is extracted again by this method, and the extract and residue are separated by filtration. The extracts obtained by the two extractions were combined and concentrated under reduced pressure to obtain a viscous dark brown liquid ethanol extract (Et Ext) of 15.0% (weight ratio) in terms of raw material. Next, the residue was extracted with 70% ethanol aqueou...

manufacture example 1

[0050] [Manufacturing example 1] Preparation example of hot water extract

[0051] The hot water extract used as a comparison in the examples was prepared by the following method. The Cordyceps sinensis mycelium powder was extracted with distilled water for 4 hours while stirring at 90-95°C. The amount of distilled water is 20mL per 1g of mycelia. The residue was filtered, and the filtrate was concentrated under reduced pressure and then freeze-dried to obtain a hygroscopic dark brown powder hot water extract (HWE).

Embodiment 2

[0052] [Example 2] Using human peripheral blood to measure immune activation effect

[0053] On a 48-well cell culture plate, 500 μL of peripheral blood collected from humans and RPMI1640 medium equal to the amount of peripheral blood were added. Add the substance to be tested so that the final concentration is 200 μg / mL, at 37 ° C, 5% CO 2 conditions for 24 hours. The culture supernatant was recovered, and interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were quantitatively measured using an enzyme-linked immunoassay (ELISA) assay kit (manufactured by BIOSOURCE). The method of using the kit and the analysis method of the quantitative results are in accordance with the operation instructions attached to the kit.

[0054] For the 50% ethanol precipitation part (50%EP), 80% ethanol precipitation part (80%EP), ethanol extract (Et Ext), 70% ethanol extract (70%Et Ext) and The hot water extract (HWE) prepared in Production Example 1 was measured for the production of IFN...

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PUM

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Abstract

[PROBLEMS] To provide a fraction containing the active ingredient contained in plant worms. To provide a means efficacious in immunopotentiation, an effective means of preventing or treating cancer and a composition useful in producing health foods. [MEANS FOR SOLVING PROBLEMS] It is intended to provide a precipitation fraction obtained by extracting plant worms mycelium with hot water and adding ethanol to the thus obtained extract. It is also intended to provide a composition for oral intake which contains the above precipitation fraction. It is further intended to provide foods, drinks, immunopotentiators, anticancer agents and so on containing the above composition for oral intake which are characterized by having an anticancer effect, an effect of preventing cancer or an immunopotentiation effect. It is furthermore intended to provide a method of effectively producing the above-described composition for oral intake.

Description

technical field [0001] The present invention relates to an isolate derived from Cordyceps sinensis having pharmacological effects and a method for producing the isolate. The present invention further relates to an oral ingestion composition, a pharmaceutical composition, a food and drink composition containing the isolate, or a medicine, quasi-drug, food and drink, etc. that have an immune activation effect and / or are used for the prevention or treatment of cancer . Background technique [0002] Cordyceps sinensis is a general term for fungi produced by insects and the like, and belongs to the genus of Ascomycota, Ergotomycetes, and Ergotaceae. Among them, Cordyceps sinensis (Berkely) Saccardo parasitizes on the larvae of the lepidopteran bat moth, and is a fungus distributed in the high mountains of China, Tibet, Nepal, and the Himalayas, and on the grasslands at an altitude of 3000-4000 meters. Since ancient times, it has been cherished and passed down as a traditional C...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61K35/64A61K31/715A61P35/00A61P37/04A61P43/00A23L1/30A23L2/52A23L33/135A61K36/00A61K36/06A61K36/07
CPCA61K31/715A61K36/068A23L1/3002A23L33/105A61P35/00A61P37/04A61P43/00
Inventor 山口能宏国友荣治内田健志
Owner KOBAYASHI PHARMA CO LTD
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