Method for looking for chemical effective parts or ingredient from traditional Chinese medicine recipe using orthogonal test

An orthogonal test design and effective part technology, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss components, etc., can solve the problems of lack of systematic research and achieve the effect of shortening the experimental cycle

Inactive Publication Date: 2006-09-27
刘凯
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Simply using the research methods of Chinese medicine chemistry and pharmacology to explore the chemically effective parts or effective material basis related to the functions and indi

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] A method for finding chemically effective parts or components in a compound Chinese medicine by utilizing an orthogonal test design, which is characterized in that: the constituent medicinal materials in the traditional Chinese medicine prescription, wherein the medicinal materials with volatile oil are extracted by steam distillation, and then each medicinal material is decocted and extracted with water respectively, Each time for 1-3 hours, add more than 10 times the amount of water, extract more than 2 times, combine multiple extracts, concentrate, and take another medicinal material containing volatile oil after extraction, the remaining decoction is concentrated, filtered, and slowly added while stirring Ethanol, so that the alcohol content reaches 70-80%, let it stand for more than 24 hours, then the supernatant part of each medicinal material will be at the same level, the precipitation part will be at the same level, the volatile oil part will be at the same level...

Embodiment 2

[0018] The above-mentioned method of using the orthogonal test design to find the chemically effective parts or components in the compound Chinese medicine, the main effective parts of the first step are taken for each medicinal material, and the ratio of the resin amount and the medicinal material amount according to the specific adsorption amount is 1:1. Porous resin column, first eluted with deionized distilled water, then eluted with 25% ethanol, then eluted with 50% ethanol, and finally eluted with ethanol, collected the eluents of each part, recovered ethanol, and concentrated aqueous solution It is multiple levels of each medicinal material, and then selects the orthogonal table according to the number of factor levels, and then prepares the test drugs of each group, and sets the original compound control group and the control group of the effective part combination of the previous step at the same time, and carries out a process that can reflect this. The pharmacologica...

Embodiment 3

[0020] The method of using the above-mentioned orthogonal test design to find the chemically effective parts or components in the compound Chinese medicine. Each medicinal material takes the main effective part of the second step, concentrates it, adjusts the pH value to 2 with hydrochloric acid, extracts it with n-butanol, and then the acid solution Use sodium hydroxide to adjust the pH value to 10, then extract with n-butanol, adjust the pH value of the extracted alkali solution to neutrality with hydrochloric acid, remove the residual solvent in the aqueous solution, and concentrate to obtain the acidic extraction and alkali extraction of each medicinal material. The three levels of the active extraction and the remaining part after extraction, and then select the orthogonal table according to the number of factor levels, and then prepare the test drugs of each group, and set up the original compound control group and the control group of the effective part combination in the...

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PUM

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Abstract

The orthogonal process for finding the effective chemical parts or components in compound Chinese medicine is disclosed, and the orthogonal process is superior to available physical and chemical analysis process. The orthogonal process includes water steam distillation to extract volatile oil, water decoction for twice or more times, concentration, filtering and other steps. The present invention is used for the separation and determination of effective components in Chinese medicine and preparation process Chinese medicine.

Description

Technical field: [0001] The invention relates to a method for finding chemically effective parts or components in traditional Chinese medicine compound prescriptions by utilizing orthogonal test design. Background technique: [0002] The chemical components in compound Chinese medicines are complex, so the research on the active ingredients and effective parts of the compound has always been a difficult point in the basic research and related application research centers of Chinese patent medicines. Simply using the research methods of Chinese medicine chemistry and pharmacology to explore the chemically effective parts or effective material basis related to the functions and indications of Chinese medicine compound prescriptions is likely to be blind and one-sided. It is an efficient idea, but there has been a lack of specific and feasible systematic research methods. Invention content: [0003] The purpose of the present invention is to provide a method for finding chem...

Claims

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Application Information

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IPC IPC(8): A61K36/00
Inventor 刘凯
Owner 刘凯
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