Cuttlebone polysaccharide CPS-1 and its preparation and use

A technology of CPS-1 and squid octopus, which is applied in the field of medicine, can solve the problems of no polysaccharide components and undetermined active ingredients

Inactive Publication Date: 2006-09-27
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its active ingredients have not been determined so far, and there are no reports about polysaccharide ingredients

Method used

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  • Cuttlebone polysaccharide CPS-1 and its preparation and use
  • Cuttlebone polysaccharide CPS-1 and its preparation and use
  • Cuttlebone polysaccharide CPS-1 and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of seaweed octopus polysaccharide CPS-1

[0026] (1) Preparation of Crude Polysaccharides from Sea Cuttlefish

[0027] Crush 5kg of sea octopus for 3 minutes, add 16L of water and cook for 10 hours, filter with gauze while it is hot, repeat the extraction of the residue twice, combine the water extracts for 3 times, concentrate to 12L with a rotary evaporator, centrifuge at 5000rpm for 10 minutes, and discard the precipitate. The supernatant was extracted three times by sevage method with a volume ratio of chloroform and n-butanol of 4:1 to remove protein, until the aqueous phase was free of protein, and then added 3 times the volume of ethanol to ethanol precipitation overnight. Discard the supernatant by centrifugation, redissolve the precipitate in distilled water, centrifuge at 5000rpm for 10 minutes, discard the insoluble matter, and precipitate the supernatant with ethanol for the second time, centrifuge to remove the supernatant, dissolve t...

Embodiment 2

[0038] Example 2: Preparation of seaweed octopus polysaccharide CPS-1

[0039]Crush 5kg of sea octopus for 5 minutes, add 12L of water and cook for 9 hours, filter with gauze while it is hot, repeat the extraction of the residue twice, combine the water extracts for 3 times, concentrate to 12L with a rotary evaporator, centrifuge at 5000rpm for 10 minutes, and discard the precipitate. The supernatant was extracted three times by sevage method with a volume ratio of chloroform and n-butanol of 4:1 to remove protein until the aqueous phase was free of protein, and then added 3 times the volume of ethanol to ethanol precipitation overnight. Discard the supernatant by centrifugation, redissolve the precipitate in distilled water, centrifuge at 5000rpm for 10 minutes, discard the insoluble matter, and precipitate the supernatant with ethanol for the second time, centrifuge to remove the supernatant, dissolve the precipitate in distilled water, and dialyze in running water for 40 hou...

Embodiment 3

[0041] Example 3: Preparation of seaweed octopus polysaccharide CPS-1

[0042] Crush 5kg of sea octopus for 8 minutes, add 14L of water and cook for 14 hours, filter with gauze while it is hot, repeat the extraction of the residue twice, combine the water extracts for 3 times, concentrate to 12L with a rotary evaporator, centrifuge at 5000rpm for 10 minutes, and discard the precipitate. The supernatant was extracted three times by sevage method with a volume ratio of chloroform and n-butanol of 4:1 to remove protein until the aqueous phase was free of protein, and then added 3 times the volume of ethanol to ethanol precipitation overnight. Discard the supernatant by centrifugation, redissolve the precipitate in distilled water, then centrifuge at 5000rpm for 10 minutes, discard the insoluble matter, and precipitate the supernatant with ethanol for the second time, centrifuge to remove the supernatant, dissolve the precipitate in distilled water, and dialyze in running water for...

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Abstract

The invention discloses a cuttle-bone polysaccharide CPS-1 and preparing method and usage in the medical domain, which is characterized by the following: boiling cuttle-bone powder to get raffinate; depositing raffinate; dialyzing; cooling; drying; getting cuttle-bone polysaccharide crude product; separating and purifying by DEAE Sepharose F.F ion exchange column, Sephacryl S-300 molecule coagulation column and Sepharose CL-6B coagulation column; getting cuttle-bone polysaccharide CPS-1. The invention can treat ulcerative colitis, which can be used for preparing the medicine of treating ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to polysaccharide CPS-1 extracted from cuttlebone octopus and its preparation method and application. Background technique [0002] Cuttlebone, also known as squid bone, is the inner shell of marine animals golden squid, needle squid or needleless squid, and is a traditional Chinese medicinal material. Its nature is salty, astringent, and warm, and it has the effects of astringent and hemostasis, astringent essence to stop belt, antacid, and astringent sores. It is widely used. It itself is used as waste in the food processing industry, a few are recycled as Chinese herbal medicines, and most of them are not well utilized. Sea octopus is often used in combination with other traditional Chinese medicines to treat various ulcers in clinic, and has good curative effect. But its active ingredient has not been determined so far, and there is no report about the polysaccharide ingredient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/04
Inventor 张建鹏冯伟华焦炳华刘军华魏江洲王俊徐红丽
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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