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Active fraction having anti-cancer and anti-metastic activity isolated from leaves and stems of ginseng

An active ingredient, active technology, applied in the field of anti-cancer and anti-metastatic active fractions isolated from ginseng leaves and stems, can solve the problem of no research showing the anti-cancer effect of ginseng leaves or stem extracts, etc., to reduce proliferation and metastasis. Effect

Inactive Publication Date: 2006-10-04
MERENS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Some studies have shown that polysaccharides obtained from ginseng leaves are effective in treating gastric ulcers (PlantaMed.58, 1992, 445--448); however, no studies have shown that ginseng leaf or stem extracts have anticancer effects, inhibit cancer metastasis, etc.

Method used

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  • Active fraction having anti-cancer and anti-metastic activity isolated from leaves and stems of ginseng
  • Active fraction having anti-cancer and anti-metastic activity isolated from leaves and stems of ginseng
  • Active fraction having anti-cancer and anti-metastic activity isolated from leaves and stems of ginseng

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0052] Preparation Example 1: Preparation of Crude Polysaccharide Extract from Ginseng Leaves and / or Stems

[0053] 100 g of leaves and / or stems of Panax ginseng C.A. Mayer were added to 1 L of water, followed by heating at 100° C. for 5 hours to extract the active ingredient therefrom. The liquid phase was separated from the leaves and / or stems by filtration and centrifugation at 7,000 rpm for 30 minutes to remove insoluble impurities therefrom. The polysaccharide-containing supernatant was concentrated to 20 Brix at 70° C. under a pressure of 760 mmHg to obtain 100 ml of a concentrate. Sodium chloride was added to a final concentration of 1 M, and 300 ml of ethanol was added. The synthesis mixture was left for 1 hour to settle. The precipitate was washed twice with 100 ml of 95% ethanol to remove impurities and then dialyzed, followed by freeze-drying to obtain a crude polysaccharide extract.

[0054] Repeat the above process of Panax quinquefolium leaves and / or stems to ...

preparation Embodiment 2

[0055] Preparation Example 2: Preparation of polysaccharides from ginseng leaves and / or stems

[0056] 500 g of Panax ginseng C.A. Mayer was added to 5 L of distilled water and heated for 3 hours to prepare an extract solution. Separate the slurry from the extract solution by gauze. The extract solution was centrifuged at 7,000 rpm for 20 minutes to separate the supernatant. The supernatant was adjusted to about pH 8.0 and 0.4M NaCl / 10mM Tris--HCl, and then loaded into an anion exchange resin (strongly basic anion exchange resin based on PA312, samyang company) at a speed of 30ml / min. The anion exchange resin had previously been equilibrated with 0.4M NaCl / 10 mM Tris-HCl in order to adsorb only impurities and prevent adsorption of polysaccharides. The anion exchange resin was washed well with buffer and the solution that had passed through the resin was adjusted to pH 6-7. The synthetic solution was loaded onto the adsorbent resin (HP 20, Mitsubishi Corporation) at a rate o...

preparation Embodiment 3

[0058] Preparation Example 3: Preparation of Extract from Ginseng Leaf and / or Stem, MB40100, and Preparation of Liquid Solution for Injection

[0059]In Preparation Example 2, the solution derived from Panax ginseng C.A.Mayer, which had been applied to the anion exchange resin column (Q-Sepharose), was applied to the column filled with silica gel to remove the causative agent therefrom. pyrogenic substance. 3 volumes of ethanol were added to precipitate polysaccharides, and the polysaccharides were collected by centrifugation and then washed twice with 95% ethanol to remove impurities. The resulting fraction was dissolved in triple distilled water and then dialyzed to remove residual ethanol with a molecular weight cut off of less than 6,000. For sterility, the obtained fractions were applied to a 0.2 μm filter system to prepare pyrogen-free solutions. The polysaccharide fraction was prepared by lyophilizing the sterile solution in 3 ml vials. Liquid solutions for injection...

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Abstract

This invention Provides a composition that contains, as an active ingredient, an extract or polysaccharides separated from plants belonging to Acanthopanax genus, for an anticancer drug, or its adjuvant having an effect on the enhancement of hemotopoiesis, defense of bone marrow, inhibition of radiation side effects, etc., and a method of preparing the extract from the Acanthopanax genus plants.

Description

technical field [0001] The present invention relates to a composition containing as an active ingredient extracted from leaves and / or stems of plants of the genus Panax and a method for preparing a composition containing polysaccharides isolated from leaves and / or stems of plants of the genus Panax, said Polysaccharides, especially polysaccharide-containing compositions, can be used as anticancer drugs and / or cancer metastasis inhibitors by activating immune cells, such as natural killer (NK) T cells, NK cells, etc., and can also enhance hematopoietic function, bone marrow defense effect, Radiosensitivity defense effect, etc. are used as adjuvants for anticancer drugs, and the present invention also relates to a method for preparing polysaccharides by extracting polysaccharides from ginseng leaves and / or stems in water at 50-180° C. for 0.5-20 hours. Background of the invention [0002] It is reported that 20% of American deaths each year are caused by cancer-related disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P35/00A61K9/08A23L1/30A61K9/10A61K9/14A61K9/20A61K9/48A61K31/191A61K31/225A61K31/337A61K31/675A61K31/7004A61K31/7012A61K31/702A61K31/715A61K33/24A61K36/00A61K36/25A61K36/254A61K45/00A61P7/00A61P7/06A61P31/04A61P31/12A61P35/04A61P37/04A61P39/00A61P43/00
CPCA23L1/3002A61K31/7004A61K31/191A61K31/225A61K31/702A61K31/7012A61K36/258A23L33/105A61P7/00A61P7/06A61P31/04A61P31/12A61P35/00A61P35/04A61P37/04A61P39/00A61P43/00A61K2300/00A61K36/254
Inventor 郭大焕申明锡金知燕朴正国
Owner MERENS CO LTD
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