Crocin finger spectrum analysis method

A crocin and analysis method technology, applied in the direction of analysis materials, material separation, measurement devices, etc., can solve the problems of drift, poor repeatability, unstable dynamic balance, etc., and achieve the effect of stable retention time and stable properties

Inactive Publication Date: 2006-12-20
JIANGSU HENGRUI MEDICINE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] According to the technical requirements of the State Food and Drug Administration for the research on the fingerprints of traditional Chinese medicine injections, when analyzing the fingerprints of crocin and its medicinal materials and injections, due to the presence of a molecule of carboxylate in the molecular structure of crocin-3 Acid (see the above formula), in the organic phase-water, will ionize H + or with H + Combining to form carboxylic acid, this dynamic equilibrium is unstable, so when the fingerprint analysis of crocin-3, the retention time of each injection is unstable, the drift is erratic, the repeatability is very poor, and it does not meet the requirements. Requirements for Fingerprint Analysis

Method used

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  • Crocin finger spectrum analysis method
  • Crocin finger spectrum analysis method
  • Crocin finger spectrum analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1314A VWD), Agilent chromatographic workstation.

[0038] When adopting the method of the present invention to measure sample 1, be filler (Shimadzu VP-ODS 150mm * 4.6mm, 5 μm) with octadecylsilane bonded silica gel; Adjust the pH to 4.0) (55:45) as the mobile phase, and the detection wavelength is 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.9-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.

Embodiment 2

[0040] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1315A DAD), Agilent chromatographic workstation.

[0041] When adopting the method of the present invention to measure sample 2, be filler (Kromasil-ODS 200mm * 4.6mm, 5 μm) with octadecylsilane bonded silica gel; The ammonium acetate solution of methanol-acetonitrile-0.2mol / L (with Acetic acid (adjusted pH4.5) (25:5:70) was used as the mobile phase, and the detection wavelength was 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.8-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.

Embodiment 3

[0043] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1314A VWD), Agilent chromatographic workstation.

[0044] When adopting the method of the present invention to measure sample 3, be filler (NOVPAK-ODS 100mm * 3.9mm, 5 μm) with octadecylsilane bonded silica gel; The sodium acetate solution of tetrahydrofuran-0.05mol / L (regulate with acetic acid pH2.8) (20:80) is the mobile phase, and the detection wavelength is 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.8-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.

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Abstract

The invention discloses a fingerprint atlas analyzing method; which is characterized by the following: adding buffer in the inversed-phase chromatogram system for west carthamin; possessing stable chemical property for west carthamin-1, west carthamin-2, west carthamin-4 in the flow phase; displaying precise quantitative property and stable reserving time for west carthamin-3; satisfying the request of fingerprint atlas analysis.

Description

technical field [0001] The invention relates to an analysis method of traditional Chinese medicine and its extract, in particular to a method for fingerprint analysis of crocin by high performance liquid chromatography. Background technique [0002] Crocin is the main component of traditional Chinese medicine saffron. In saffron extract and crocin for injection, its main compounds include crocin-1, crocin-2, saffron Glycoside-3, crocin-4, their specific chemical structural formula and molecular formula are as follows: [0003] [0004] Crocin-1 formula C 44 h 64 o 24 [0005] [0006] Crocin-2 formula C 38 h 54 o 19 [0007] [0008] Crocin-3 Formula C 30 h 42 o 14 [0009] [0010] Crocin-4 Formula C 26 h 34 o 8 [0011] It is recorded in the prior art that crocin has the effects of promoting blood circulation and removing blood stasis, and loosening knots; [0012] The fingerprint test method of the prior art is carried out according to the hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/60G01N30/02
Inventor 李语如
Owner JIANGSU HENGRUI MEDICINE CO LTD
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