Crocin finger spectrum analysis method
A crocin and analysis method technology, applied in the direction of analysis materials, material separation, measurement devices, etc., can solve the problems of drift, poor repeatability, unstable dynamic balance, etc., and achieve the effect of stable retention time and stable properties
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Embodiment 1
[0037] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1314A VWD), Agilent chromatographic workstation.
[0038] When adopting the method of the present invention to measure sample 1, be filler (Shimadzu VP-ODS 150mm * 4.6mm, 5 μm) with octadecylsilane bonded silica gel; Adjust the pH to 4.0) (55:45) as the mobile phase, and the detection wavelength is 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.9-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.
Embodiment 2
[0040] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1315A DAD), Agilent chromatographic workstation.
[0041] When adopting the method of the present invention to measure sample 2, be filler (Kromasil-ODS 200mm * 4.6mm, 5 μm) with octadecylsilane bonded silica gel; The ammonium acetate solution of methanol-acetonitrile-0.2mol / L (with Acetic acid (adjusted pH4.5) (25:5:70) was used as the mobile phase, and the detection wavelength was 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.8-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.
Embodiment 3
[0043] Instruments Agilent 1100 high pressure liquid chromatography (including G1312A BinPump, G1313AALS, G1314A VWD), Agilent chromatographic workstation.
[0044] When adopting the method of the present invention to measure sample 3, be filler (NOVPAK-ODS 100mm * 3.9mm, 5 μm) with octadecylsilane bonded silica gel; The sodium acetate solution of tetrahydrofuran-0.05mol / L (regulate with acetic acid pH2.8) (20:80) is the mobile phase, and the detection wavelength is 440nm. Adjust the flow rate so that the retention time of the crocin-1 peak is 2.8-3.3 minutes, and the number of theoretical plates calculated based on the crocin-1 peak should not be less than 1500.
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