Application of Rhabditis (Oscheius) spp. in control of plant nematode
A technology for small rod nematodes and plant nematodes, applied in the fields of application, nematicides, plant growth regulators, etc., to achieve the effects of easy cultivation and propagation, convenient use, and easy acquisition
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[0020] 1 Materials and methods
[0021] 1.1 Test material
[0022] The test agent: the small rod nematode Rhabditis (Oscheius) sp. (Bn) was lured by the lab members from the cucumber rhizosphere soil in the greenhouse with mature larvae of the greater wax moth, and the mature larvae of the greater wax moth were used to separate, purify, and amplify It can be propagated and cultivated, and can be released to the public. The collected small rod nematodes were suspended in the aqueous solution, stored at 4°C by the shallow water layer method, and administered within 20 days.
[0023] 1.2 Test site
[0024] This experiment was carried out in a greenhouse in Liancheng Township, Tongxu County, Henan Province. The greenhouse runs east-west, 50m long and 8m wide, and is divided into 40 north-south ridges. The cross-section of the ridge is saddle-shaped, and the saddle valley is used for watering. Two rows of cucumbers are planted on both sides, with about 40 plants in each row. Th...
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