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Low-density lipoprotein cholesterol quantitative determining method reagent and reagent kit

A technology for quantitative determination of low-density lipoprotein, which is applied in the field of quantitative determination of low-density lipoprotein cholesterol and can solve the problem of high price

Active Publication Date: 2007-01-03
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the commercially available reagents currently used for direct determination of LDL-C content are all imported products, and the price is relatively high

Method used

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  • Low-density lipoprotein cholesterol quantitative determining method reagent and reagent kit
  • Low-density lipoprotein cholesterol quantitative determining method reagent and reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] One, prepare following reagent I and reagent II of the present invention according to following composition and ratio:

[0038] Reagent I:

[0039] MOPS buffer, pH7.0 30mM

[0040] 4-Aminoantipyridine 0.1g / L

[0041]Cholesterol esterase (CEH) 1520u / L

[0042] Cholesterol oxidase (COD) 1070u / L

[0043] Peroxidase (POD) 8000u / L

[0044] Agricultural Emulsion No. 601 5g / L

[0045] NaN 3 1g / L

[0046] Reagent II:

[0047] MOPS buffer, pH7.0 30mM

[0048] Color developer TOOS 0.2g / L

[0049] NaN 3 1g / L

[0050] TRITON X-100 20g / L

[0051] 2. Mix 300 μl reagent I and 3 μl serum sample in the sample tube, incubate at 37°C for 5 minutes, use HITACHI7060 automatic biochemical analyzer, measure the absorbance A at the main wavelength of 600nm and the secondary wavelength of 700nm 1 , then add 100 μl reagent II to the sample, mix well, incubate at 37°C for 5 minut...

Embodiment 2

[0064] Using the reagent of Example 1 of the present invention, the LDL-C content in 11 healthy human serum samples was determined under the conditions described in Example 1. For the same sample, a commercially available precipitation method kit (low-density lipoprotein cholesterol (LDL-C) polyethylene sulfate precipitation method kit, batch number: Zhongsheng 210181, produced by Zhongsheng Beikong Biotechnology Co., Ltd.) was used for comparison Quantitative determination. The results are shown in Table 2 below.

[0065]

sample number

LDL-C (mg / dl)

The method of the invention

Precipitation

1

85

87

2

79

70

[0066] 3

111

112

4

97

93

5

130

118

6

88

89

7

73

70

8

253

263

9

120

129

10

143

133

11

80

91

...

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Abstract

A reagent for the content of the low density lipoprotein cholesterol in the quantitative determining serum sample, consists of the reagent I and reagent II that places respectively, the reagent I contains the styrylphenol polyoxyethylene ether and the quantitative enzyme reagent; the reagent II contains the reaction accelerant. The invention provides a reagent box with the reagent I and the reagent II, and a method for determining the low density lipoprotein cholesterol content.

Description

technical field [0001] The invention relates to a quantitative determination method, reagent and kit for low-density lipoprotein cholesterol (LDL-C). Background technique [0002] Lipids such as cholesterol cooperate with apoproteins in serum to form lipoproteins. Lipoproteins are classified into chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), etc. according to their physical properties. Since many epidemiological, genetic and clinical studies have confirmed that serum low-density lipoprotein cholesterol (LDL-C) levels are positively correlated with the incidence of atherosclerosis and coronary heart disease, high LDL-C is usually the primary cause of coronary heart disease. For this reason, the determination of LDL-C has been paid more and more attention in clinical practice. Therefore, the determination of LDL-C has an important predictive role in the incidence of cardiovascular disease. [0003] Cli...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/92
Inventor 张瑛王淑娟
Owner BIOSINO BIO TECH & SCI
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