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Regulation of sperm function

A sperm capacitation and sperm technology, applied in the field of regulation of sperm function, can solve problems such as fertility damage

Inactive Publication Date: 2007-01-10
QUEEN MARY UNIV OF LONDON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, depending on the species and individual, there are varying degrees of damage associated with sperm freezing and thawing, thus compromising their fertility compared to fresh, unfrozen sperm

Method used

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  • Regulation of sperm function
  • Regulation of sperm function
  • Regulation of sperm function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Thawed sperm samples were incubated with supplements as follows:

[0060] 1. Incubate sperm with fibronectin (2 μg / l) for 10 minutes.

[0061] 2. Combine sperm with fibronectin (2 μg / l) plus RGDS (5×10 -6 mol / L) together for 10 minutes.

[0062] 3. Angiotensin II (10 -9 mol / L) was added to fibronectin plus RGDS samples and incubated for an additional 10, 20, 30 and 40 minutes.

[0063] All incubations were performed at 37°C with a water bath and heated stage.

[0064] Semen samples (10 [mu]l) were applied to pre-warmed (37[deg.]C) glass slides, placed on the heated stage of a microscope and examined. The number of motile spermatozoa was counted in the control sample at time zero and in the treated samples after the incubation time.

[0065] result in figure 1 is given in , where the bars show the mobility values ​​at the following moments:

[0066] 1. Control 0min;

[0067] 2. Fibronectin for 10 minutes;

[0068] 3. Fibronectin + RGD 10min;

[0069] 4. Fibrone...

Embodiment 2

[0081] Thawed sperm samples were incubated with supplements as follows:

[0082] 1. Incubate sperm with fibronectin (2 μg / l) for 10 minutes.

[0083] 2. Angiotensin II (10 -9 mol / L) was added to the fibronectin samples and incubated for an additional 10, 20 and 30 minutes.

[0084] result in figure 2 is given in , where the bars show the mobility values ​​at the following moments:

[0085] 1) Control 0min;

[0086] 2) Fibronectin for 10 minutes;

[0087] 3) Fibronectin + angiotensin II for 10 minutes;

[0088] 4) Fibronectin + angiotensin II for 20 minutes;

[0089] 5) Fibronectin + angiotensin II for 30 minutes;

[0090] 6) After 30 minutes of control;

[0091] It can be seen from the figure:

[0092] a) The control sample showed 79% motility at time zero;

[0093] b) The effect of fibronectin concentration on spermatozoa shows very significant changes in total sperm motility. Only 8% of the sperm motility was present after 5-10 minutes of incubation.

[0094] b)...

Embodiment 3

[0097] Thawed sperm samples were incubated with supplements as follows:

[0098] 1. Combine the sperm with RGDS (5×10 -6 mol / L) together for 5 minutes.

[0099] 2. Add fibronectin (2 μg / l) to RGD S samples and incubate for another 5 min.

[0100] 3. Angiotensin II (10 -9 mol / L) was added to the fibronectin+RGDS samples and incubated for another 10, 20 and 30 minutes.

[0101] result in image 3 is given in , where the bars show the mobility values ​​at the following moments:

[0102] 1) Control 0min;

[0103] 2) Pre-incubate with RGD for 5 minutes;

[0104] 3) RGD+fibronectin for 5min;

[0105] 4) Fibronectin + RGD + angiotensin II for 10 minutes;

[0106] 5) Fibronectin + RGD + angiotensin II for 20 minutes;

[0107]6) Fibronectin + RGD + angiotensin II for 30 minutes;

[0108] 7) Control 30min;

[0109] It can be seen from the figure:

[0110] a) The control sample showed 62% motility at time zero;

[0111] b) Pre-incubation of RGDS with sperm showed a 19% reduc...

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Abstract

An extracellular matrix protein, such as fibronectin, vitronectin or laminin, is added to a sperm sample to bring the sperm into a low motility, non-capacitated state or maintain a pre-existing non-capacitated state. Subsequently, at an appropriate time in an in vitro fertilization study or in vivo fertilization procedure, angiotensin II or a related peptide is added to the sperm sample to enhance motility and capacitate the sperm. The extracellular matrix protein prevents undesirable early natural capacitation, and artificial insemination procedures can be made more precise, as sperm can be brought to the right state of capacitation for transfer at precisely the right time. The treatment with angiotensin II can be combined with use of the peptide RGD to compete with the binding of the extracellular matrix protein and so suppress its effect.

Description

technical field [0001] The present invention relates to the regulation of sperm function. The present invention particularly relates to the use of specific proteins such as fibronectin and angiotensin II for preserving non-capacitated or non-activated spermatozoa and converting non-capacitated / non-activated spermatozoa into capacitated / activated states, respectively. Background technique [0002] The physiological factors that induce and maintain maturation and motility of mammalian spermatozoa are generally still unclear, although various agents are known to be involved. For example, data on motility of stimulated and unstimulated sperm from volunteers and patients attending fertility clinics showed that angiotensin II increased both the percentage of motile sperm and their linear velocity, while specific ATI receptors The antagonist losartan inhibits the effect of angiotensin II on the percentage of motile sperm. Angiotensin II has been shown to have an effect on specifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/09A61K38/39A61P15/08A61K35/52C12N5/076
CPCC12N5/061A61K38/09A61K35/52A61P15/08A61P43/00A61K2300/00
Inventor 加文·文森
Owner QUEEN MARY UNIV OF LONDON
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