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Angelica dehurica water extractive liquid fingerprint, its establishmetn method and application

A fingerprint spectrum and a technology for establishing a method are applied in the field of the fingerprint spectrum of the water extract of Angelica dahurica and its establishment, which can solve problems such as no reports, and achieve the effects of good stability and high repeatability.

Inactive Publication Date: 2007-01-31
JINAN UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] At present, the fingerprints of the water extract of Angelica dahurica have not been reported

Method used

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  • Angelica dehurica water extractive liquid fingerprint, its establishmetn method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Extraction of Angelica dahurica medicinal materials: Accurately weigh 10g of Angelica dahurica powder, add 150ml of deionized water, decoct for 10min, filter with ordinary filter paper, concentrate the filtrate to 100ml, pass through a 0.45um filter membrane, and use the filtrate as the test solution for HPLC analyze. Prepare the test solution of Angelica dahurica medicinal materials from 13 different origins (see Table 6) respectively.

[0036] (2) Establishment of fingerprints: Precisely draw 20ul of the test solution, and measure it by high performance liquid chromatography, using methanol-water as the mobile phase system, column temperature 30°C, wavelength 254nm, 0-5min, 5%-20% Methanol; 5 ~ 15min, 20% methanol; 20 ~ 30min, 20% ~ 70% methanol; 20 ~ 30min, 70% methanol; 30 ~ 40min, 70% ~ 90% methanol, the above methanol is calculated by volume percentage; flow rate 1ml / min, Lichrospher5-C18 (250mm × 4.6mm) chromatographic column, recording time 40min is the be...

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Abstract

A fingerprint for the aqueous extract of dahurian angelica root in order to test the chemical components in dahurian angelica root, and discriminate the quality of dahurian angelica root is created through extracting to obtain aqueous extract, creating its HPLC fingerprint, and testing 13 batches of dahurian angelica root with different origin places to find out 11 common peaks.

Description

technical field [0001] The invention relates to a fingerprint, in particular to a fingerprint of the water extract of the medicinal material Angelica dahurica and its establishment method and application. Background technique [0002] Traditional Chinese medicine is composed of multiple components that work on multiple targets, and the current quality control standard uses a specific component as an indicator. With the modernization of Chinese medicine research, the quality research of Chinese medicine has gradually changed from a single active component or a qualitative index component. Quantitative analysis turned to group analysis of effective components. At present, the quality control of Angelica dahurica is limited to the content determination of imperatorin and isoimperatorin in Angelica dahurica. Therefore, in the quality control of Angelica dahurica medicinal materials, in addition to establishing the content determination method of the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/232G01N30/02G01N30/88
Inventor 聂红张慧罗勇
Owner JINAN UNIVERSITY
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