Method for rat body temperature detection by using thermo-scan image-forming system
A technology for experimental rats and imaging systems, applied in applications, thermometers, measuring devices, etc., can solve problems such as poor repeatability, time-consuming, labor-intensive, and easy damage to measuring organs, and achieve easy data reading, short scanning time, Informative effect
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Embodiment 1
[0021] Put the thermal scanning imaging system in a clean environment, turn on the instrument switch, and put it in the state to be tested. Evenly spread a layer of 1-2cm thick absorbent cotton on the bottom of the glass desiccator, pour the anesthetic ether until it is just submerged in the absorbent cotton, place the loading plate above it stably, put the experimental rats to be tested on the loading board, and cover tightly Desiccator, the rat was anesthetized by ether inhalation. After the rat was lying still for 10 seconds due to anesthesia, it was quickly taken out of the desiccator, fixed on a fixed plate on its back (fixation time was 15 seconds), and then placed in front of the scanning lens of the thermal scanning imaging system for scanning . In addition, an ordinary thermometer was used to measure the axillary and rectal temperatures of the rats according to the conventional method. The results of the two methods are shown in Table 1.
[0022] Measured...
Embodiment 2
[0026] The specific determination steps are the same as those described in Example 1, except that the resting and immobilization time of several rats to be tested due to anesthesia are controlled at different levels (see Table 2 for details). As can be seen from Table 2, it is very important to control the proper lying down and fixed time. If the time of rats lying still due to anesthesia is less than 10 seconds, the degree of anesthesia is too shallow, and they are awake and struggling during scanning, which affects the scanning measurement; if the time of rats lying still due to anesthesia is more than 30 seconds, The degree of anesthesia is too deep, and the body temperature appears to drop. On the premise of ensuring that the rat can be fixed, the immobilization time of the rat is controlled within 30 seconds at the longest, which varies according to the resting time of the rat. It can be seen that the time for the control rats to lie still due to anesthesia is within 10 ...
Embodiment 3
[0029]Table 3 is the comparison of the anesthesia mode adopted by the method of the present invention and the intravenous injection of chloral hydrate anesthesia mode (with the common thermometer measurement method as contrast), and the specific measurement steps are described in Example 1. The time for lying still under anesthesia was 10 seconds, and the fixed time was 30 seconds. The results show that the body temperature measured by the method of the present invention is not significantly different from the value measured by the common thermometer after anesthetized rats with ether or halothane, and the deviation between several measurement results is smaller than that of the common thermometer method. However, after anesthesia by intravenous injection of chloral hydrate, the body temperature measured by the thermal scanning imager is slightly lower than the value measured by the ordinary thermometer, and the deviation between several measurement results is smaller than that...
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