Application of doxycycline ad medicine for promoting cell proliferation

A cell proliferation, doxycycline technology, applied in the direction of drug combination, tetracycline active ingredients, etc., can solve problems such as inability to relieve pulmonary hypertension

Inactive Publication Date: 2007-02-14
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0025] Vieillard-Baron reported that MMPs are involved in the formation of pulmonary hypertension but inhibition of some MMPs does not relieve pulmonary hypertension

Method used

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  • Application of doxycycline ad medicine for promoting cell proliferation
  • Application of doxycycline ad medicine for promoting cell proliferation
  • Application of doxycycline ad medicine for promoting cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Proliferation-promoting effect of doxycycline on mouse thymus medullary epithelial cell line MTEC1

[0046] When doxycycline is used as the inducer of the Tet-on (tetracycline-induced promoter system) system to induce the expression of related genes in the MTEC1 cell line, use 3 Cell proliferation was measured by the H-TdR incorporation assay. The specific steps are as follows: After washing the cells of the mouse thymus medullary epithelial cell line MTEC1 in the logarithmic growth phase, they were suspended in 5×10 cells with DMEM complete medium. 4 / ml, inoculated in a 96-well culture plate, 100 μl per well, respectively added doxycycline 0.1, 1, 10 and 100 μg / ml to the cell culture, and set up a control group (without doxycycline). Each treatment group made 6 duplicate wells, and each concentration treatment group was cultured for 24, 48, and 72 hours respectively, and 0.5 μCi / well was added 12 hours before cell harvest. 3 H-TdR, continue to culture to ...

Embodiment 2

[0053] Example 2. Proliferation-promoting effect of doxycycline on other cells

[0054] Contrary to our results, many literatures reported that doxycycline can inhibit the proliferation and induce apoptosis of many tumor cells, activated T and B cells. Under the same experimental conditions as in Example 1, we used 3 The effect of doxycycline on cell lines from different sources was detected by H-TdR incorporation method. Such as image 3 and Figure 4 As shown, doxycycline can promote the proliferation of HEK293, CTX-3, TEC1C8, TNC-R3.1 and mouse fresh thymic stromal cells, but the effect on these cells is weaker than that of MTEC1; on the contrary, it can Inhibits Con A-activated T cells, T lymphoma cell lines (Jurkat, C2 and EL-4) and LPS-activated B cells; as image 3 As shown, doxycycline has no obvious pro-proliferative effect on HUVEC cell lines. In addition, these effects are dose-dependent. (Note: ConA is concanavalin A, LPS is lipopolysaccharide)

Embodiment 3

[0055] Example 3. Morphological observation of the effect of doxycycline on promoting the proliferation of MTEC1 cells

[0056] In order to further confirm the role of doxycycline in promoting the proliferation of MTEC1 cells, we used the method of morphological observation. 3×10 5 / ml (about 3.12×10 4 / cm 2 ) MTEC1 cells were inoculated in 6-well cell culture plates, 1ml per well, added different concentrations of doxycycline and cultured for 72 hours, and a blank control was set up at the same time, with three replicate wells in each group, observed and photographed under an inverted microscope. Such as Figure 5 As shown, after MTEC1 cells were cultured with different concentrations of doxycycline for 7 days, the cells in the control group shown in group A died; in group B, a small number of cells cultured with 0.1 μg / ml doxycycline began to die; in group C, 1 μg / ml doxycycline The cells cultured with 1 ml doxycycline continued to grow; in group D, the cells cultured wi...

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Abstract

An application of doxycycline in promoting the reproduction of cells, such as the cells MTEC1, TEC1C8, TNC-R3.1, HEK293, CTX-3, etc, is disclosed.

Description

technical field [0001] The present invention relates to the use of tetracyclines, in particular to the use of doxycycline as a drug for promoting cell proliferation. Background technique [0002] Doxycycline is a tetracycline antibiotic, which is widely used clinically in the treatment of bacterial and protozoan infections. In recent years, it has been found that doxycycline and other tetracycline derivatives have other biological effects, and are currently being experimentally used in the clinical treatment of tumors , autoimmune diseases, periodontal disease, cerebral ischemia and hormone replacement therapy in postmenopausal women. [0003] 1. Structure and antibacterial mechanism of doxycycline and other tetracyclines [0004] Tetracycline drugs mostly have a similar four-ring core structure-hydrogenated tetraphenyl, and the different derivatives are only different in the substituting groups. Tetracyclines can be divided into natural products and semi-synthetic product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/65A61P43/00
Inventor 陈慰峰邵启祥张毓
Owner PEKING UNIV
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