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Paraffin section method for seawater fish egg

A paraffin section, sea fish technology, applied in sampling devices, preparation of test samples, etc., can solve the problems of egg deformation, voids, discontinuous wax slices, etc., to simplify procedures, improve work efficiency, and easily obtain medicines. Effect

Inactive Publication Date: 2007-02-14
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method capable of improving and improving the quality of paraffin sections of seawater fish eggs. Through the implementation of this method, the time-consuming step of stripping off the egg membranes can be omitted, the effect of paraffin sections of seawater fish eggs can be simplified and improved, and the problem of seawater can be solved. Deformation of eggs, discontinuity of wax slices and cavities during paraffin sectioning of fish eggs

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Embodiment Construction

[0019] Describe technical method of the present invention in detail below in conjunction with accompanying drawing by embodiment:

[0020] Paraffin sections include: fixation, dehydration, clearing, paraffinization, embedding, sectioning, dewaxing, staining, clearing and mounting. The present invention improves the fixative, dehydration procedure, transparent procedure, paraffin penetration method, embedding procedure and dyeing method in the paraffin section process of seawater fish eggs, that is, the selection of the fixative, the determination of the dehydration procedure, and the transparency procedure are carried out. The determination of the wax penetration procedure, the improvement of the dyeing method.

[0021] Choice of fixative: Commonly used light microscope sample fixatives are not ideal for fixing seawater fish egg samples, and slices often break during the slicing process. We used glutaraldehyde, which is commonly used for electron microscope sample fixation, a...

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Abstract

A method for preparing paraffin section of seawater roe includes selection of fixing agent, confirmation of dewatering process, confirmation of transparent process, confirmation of wax penetration process, improvement of dyeing mode. In the method, egg capsule stripping process being difficultly operated is omitted.

Description

technical field [0001] The invention belongs to a seawater fish egg paraffin section technology, and is a method for comprehensively improving fixative, dehydration procedure, transparent procedure, wax penetration method and dyeing method on the basis of the existing paraffin section technology. Background technique [0002] Before the present invention was made, paraffin section was a traditional biological research technique due to its simple operation and low price. Paraffin section included: fixation, dehydration, transparency, paraffin penetration, embedding, sectioning, patching, dewaxing , staining, clearing, and mounting are widely used in many fields of biology. However, due to the special egg membrane structure of seawater fish eggs, the effect of paraffin sectioning has been unsatisfactory. For freshwater fish eggs, the traditional method of paraffin sectioning after peeling off the egg membrane has been adopted at home and abroad, and the effect has been signifi...

Claims

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Application Information

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IPC IPC(8): G01N1/06G01N1/28
Inventor 张岩张立敬黄倢陈四清肖永双
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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