Method for biologic preparing 4, 4 dihydric bowel lactone from burdock glycoside
A technology of hydroxyenterolactone and arctiin, which is applied in the direction of drug combination, diseases, bone diseases, etc., can solve the problems of low conversion rate and unsuitable enterolactone, etc., and achieve high theoretical value and economic benefits
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Embodiment 1
[0021] 1. Strain cultivation:
[0022] Add 0.2 mg of arctiin to 20 mL of GAM broth medium, and then add 0.02 g of healthy 35°C anaerobic culture for 24 hours to obtain a culture solution. 10 mL of the culture solution was added to 90 mL of GAM broth medium containing 1.0 mg arctiin, and anaerobically cultured at 35° C. for 24 hours to obtain a bacterial culture solution, which was used as a strain.
[0023] 2. Preparation of 4,4'-dihydroxyenterolactone:
[0024] Add 50 mg of arctiin to 900 mL of GAM broth medium, then add 100 mL of the strain prepared in the above step (1), and culture anaerobically at 35° C. for 1 day. Extract with ethyl acetate, concentrate the extract under reduced pressure, separate and purify through a silica gel column to obtain 20 mg of 4,4'-dihydroxyenterolactone.
Embodiment 2
[0026] 1. Strain cultivation:
[0027] Add 2 mg of arctiin to 10 mL of GAM broth medium, then add 1.0 g of fresh feces from a healthy person, and culture it anaerobically at 37° C. for 24 hours to obtain a culture solution. 10 mL of this culture solution was added to 100 mL of GAM broth medium containing 20 mg arctiin, and cultured anaerobically at 37° C. for 36 hours. Then 100 mL of the culture solution was added to 400 mL of GAM broth medium containing 100 mg of arctiin, and anaerobically cultured at 37° C. for 36 hours to obtain 500 mL of a bacterial culture solution with enhanced activity. This bacterial culture solution was used as a strain.
[0028] 2. Preparation of 4,4'-dihydroxyenterolactone:
[0029] Add 500 mg of arctiin to 500 mL of GAM broth medium, then add 500 mL of the strain prepared in the above step (1), and culture anaerobically at 37° C. for 5 days. Extract with ethyl acetate, concentrate the extract under reduced pressure, separate and purify through a...
Embodiment 3
[0031] 1. Strain cultivation:
[0032] Add 10 mg of arctiin to 10 mL of GAM broth medium, then add 2.0 g of fresh feces from healthy people, and culture at 40° C. for 24 hours to obtain a culture solution.
[0033] 10 mL of the culture solution was added to 40 mL of GAM broth medium containing 40 mg arctiin, and cultured anaerobically at 40° C. for 48 hours. Then 50 mL of the culture solution was added to 50 mL of GAM broth medium containing 50 mg of arctiin, and cultured anaerobically at 37° C. for 48 hours. Then 100 mL of the culture solution was added to 100 mL of GAM broth medium containing 100 mg arctiin, and cultured anaerobically at 37° C. for 48 hours. Then 200 mL of the culture solution was added to 200 mL of GAM broth medium containing 200 mg of arctiin, and cultured anaerobically at 37° C. for 48 hours. Then 400 mL of the culture solution was added to 400 mL of GAM broth medium containing 400 mg arctiin, and anaerobically cultured at 37° C. for 48 hours to obtain ...
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