Preventive and/or therapeutic agent for cachexia

a technology of therapeutic agent and cachexia, which is applied in the direction of metabolism disorder, extracellular fluid disorder, peptide/protein ingredient, etc., can solve the problems of shortening life, lowering the defensive function of the biological body, and insufficient alimentation treatmen

Inactive Publication Date: 2002-04-11
DAIICHI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further, if radiotherapy and / or chemotherapy is carried out for a patient with progressive or terminal cancer for whom curative operation can not be expected, it may lead to extremely lowered biological body defensive function such as immunological function due to specific malnutrition, resulting in shortening life.
Therefore, these are serious problems in practical treatment thereof.
However, in cachexia, intake of energy will be used not for saving patient's life but for proliferation of tumor cell, so that alimentation can not be sufficient treatment.

Method used

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  • Preventive and/or therapeutic agent for cachexia
  • Preventive and/or therapeutic agent for cachexia
  • Preventive and/or therapeutic agent for cachexia

Examples

Experimental program
Comparison scheme
Effect test

manufacturing example 1

[0021] Purification of TCF-II

[0022] According to a method described in WO 90 / 10651 and a method of Higashio et al (Higashio, K. et. al, B. B. R. C., vol.170, pp397-404 (1990)), cell was cultured and purified TCF-II was obtained. That is, 3.times.10.sup.6 human fibroblast IMR-90 (ATCC CCL-186) cells were placed in a roller bottle containing 100 ml DMEM medium including 5% calf fetal serum and cultured by rotating it at the rate of 0.5-2 rpm for 7 days. When the total number of cell reached 1.times.10.sup.7, cells were deprived from the wall by tripsin digestion and collected at the bottom of bottle. And 100 g of ceramic with the size of 5-9 mesh (Toshiba Ceramic) was sterilized and put therein, which was cultured for 24 hours. After then, 500 ml of the above culture medium was added thereto and the culture was continued. The total volume of culture medium was recovered every 7-10 days and fresh medium was supplemented. Production was kept for 2 months like this and 4 liters of cultur...

manufacturing example 2

[0023] Production of Recombinant TCF-II

[0024] According to the method described in WO 92 / 01053, cell transformed with TCF-II gene was cultured and purified TCF-II was obtained. That is, transformed Namalwa cell was cultured and 20 l of culture medium was obtained. This culture medium was treated by CM-sphadex C-50 chromatography, Con-A Sepharose CL-6B chromtography and finally HPLC equipped with a Mono S column to yield about 11 mg of recombinant TCF-II.

manufacturing example 3

[0025] Manufacturing of Pharmaceutical Preparation of TCF-II

[0026] An example of manufacturing injections of TCF-II obtained in example 1 and 2 was shown.

1 (1) TCF-II 20 .mu.g human serum albumin 100 mg

[0027] The above composition was dissolved in citric acid buffer solution with pH 6.03 so that the total volume would be 20 ml. Then it was divided into vials containing 2 ml each after sterilization and sealed after lyophilization.

2 (2) TCF-II 40 .mu.g Tween 80 1 mg human serum albumin 100 mg

[0028] The above composition was dissolved in physiological saline solution for injections so that the total volume would be 20 ml. Then it was divided into vials containing 2 ml each after sterilization and sealed after lyophilization.

3 (3) TCF-II 20 .mu.g Tween 80 2 mg Sorbitol 4 g

[0029] The above composition was dissolved in citric acid buffer solution with pH 6.03 so that the total volume would be 20 ml. Then it was divided into vials containing 2 ml each after sterilization and sealed after ...

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Abstract

The present invention provides an agent for preventing and/or treating cachesia comprising TCF-II as an effective ingredient. An excellent agent for preventing and treating cachexia caused by cancer, acquired immunodeficient syndrome (AIDS), cardiac diseases, infectious disease, shock, burn, endotoxinemia, organ inflammation, surgery, diabetes, collagen diseases, radiotherapy, chemotherapy is provided by the present invention and useful for medicine.

Description

[0001] The present invention relates to an agent for preventing and / or treating cachexia comprising Tumor Cytotoxic Factor-II(TCF-II) as an effective ingredient. An excellent agent for preventing and treating cachexia caused by one of the factors selected from the group consisting of cancer, acquired immunodeficient syndrome (AIDS), cardiac diseases, infectious disease, shock, burn, endotoxinemia, organ inflammation, surgery, diabetes, collagen diseases, radiotherapy, chemotherapy is provided by the present invention and useful for medicine.BACKGROUND TECHNOLOGY[0002] Generally, such a disease as cancer, acquired immunodeficiency(AIDS), cardiac disease etc. will accompany with anorexia, weight loss, physical exhaustion, marasmus, dermatrophia, xerosis, anemia, edema, abnormal blood coagulation-fibrinolysis etc. and these pathology is defined as cachexia. After suffering from this systemic marasmus, a patient will eventually die (Tamaguma, M. et. al., Igakuno-ayumi, 149, 371-373(1989...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/22A61K38/18
CPCA61K38/1833A61P3/02A61P7/06
Inventor KOJIRO, MASAMICHIYANO, HIROHISAIEMURA, AKIHIRO
Owner DAIICHI PHARMA CO LTD
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