Wet-dry cleaning system
a technology of wet-dry cleaning and cleaning system, which is applied in the field of wet-dry cleaning system, can solve the problems of user difficulty in determining which option or specific combination of options will provide maximum cleaning without unreasonable effort and expense, and achieve the effect of reducing the burden on the user
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example 1
[0044] Extraction From Skin-like Material After Cleaning
[0045] Vitro-Corneum.TM. was cut into 5 cm .times.5 cm (.+-.1.5 cm) squares using scissors. 10 .mu.l of the 10 .mu.m FITC labeled carboxlyate-modified beads were pipetted onto each piece of Vitro-Corneum.TM. The pieces of Vitro-Corneum.TM. were placed in a hydration chamber (IMS, Inc., Milford, Conn.) overnight. The following day, samples of the materials to be used for wiping were cut into approximately 13 cm .times.5 cm pieces (.+-.1.5 cm). Cottonelle.RTM. Fresh.TM. Rollwipes samples (KIMBERLY-CLARK CORPORATION, Neenah, Wis.) were placed into sealed plastic bags after preparation to prevent the test product from drying out prior to testing. Additionally, each piece of Vitro-Corneum.TM. was removed from the hydration chamber immediately before testing. The remaining pieces of the Vitro-Corneum.TM. were left in the chamber to remain hydrated.
[0046] To ensure that each test material hasuniform contact time and pressure with the ...
example 2
[0048] Flow Cytometry Analysis of Fluorescent Beads
[0049] The flow cytometry analyses were performed on a FACSCALIBUR flow cytometer (BECTON DICKINSON BIOSCIENCES, San Jose, Calif.). equipped with a 15-mW, 488-nm, air-cooled argon ion laser. Samples were delivered at the low flow rate, corresponding to 12 .mu.l (.+-.2 .mu.l) per minute. Forward scatter, side scatter, and one fluorescent signal were measured. A band pass filter of 530 nm (515 to 545 nm) was used to collect the FITC fluorescence. Forward scatter was collected with a diode detector. Side scatter was collected with a photomultiplier tube. Using the CELLQUEST version 3.1 software (BECTON DICKINSON), the acquired data was analyzed and the desired bead population was counted.
example 3
[0050] Comparative Results From Cleaning Processes
[0051] Cleaning processes utilizing three wiping steps were performed for all combinations of wet and dry wiping. A cleaning process utilizing one wipe with a portion of KIMWIPES.RTM. EX-L-Wipers was performed as a control. Each process was repeated 14-15 times, and the results were averaged to provide the percentage of fluorescent beads removed.
[0052] The following parameters were calculated from the fluorescent imaging analysis after the three-step cleaning procedures were complete, and are given in Table A:
[0053] 1) ratio of beads removed to beads applied;
[0054] 2) ratio of beads removed by wet-dry process to beads removed by control process; and
[0055] 3) ratio of beads remaining after wet-dry process to beads remaining after control process.
1TABLE A beads removed / beads removed / beads remaining / Process* beads applied (%) control control ddd 53.7 2.451 0.593 ddw 79.8 3.803 0.255 dww 81.5 3.507 0.241 wwd 83.9 3.611 0.210 wdw 86.8 ...
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