Protein scaffolds for antibody mimics and other binding proteins

Inactive Publication Date: 2005-11-17
BRISTOL MYERS SQUIBB CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0007] The present invention provides a new family of proteins capable of evolving to bind any compound of interest. These proteins, which make use of a fibronectin or fibronectin-like scaffold, function in a manner characteristic of natural or engineered antibodies (that is, polyclonal, monocl

Problems solved by technology

These two loops have been randomized and products selected for antigen binding, but thu

Method used

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  • Protein scaffolds for antibody mimics and other binding proteins
  • Protein scaffolds for antibody mimics and other binding proteins
  • Protein scaffolds for antibody mimics and other binding proteins

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Embodiment Construction

[0053] The novel antibody mimics described herein have been designed to be superior both to antibody-derived fragments and to non-antibody frameworks, for example, those frameworks described above.

[0054] The major advantage of these antibody mimics over antibody fragments is structural. These scaffolds are derived from whole, stable, and soluble structural modules found in human body fluid proteins. Consequently, they exhibit better folding and thermostability properties than antibody fragments, whose creation involves the removal of parts of the antibody native fold, often exposing amino acid residues that, in an intact antibody, would be buried in a hydrophobic environment, such as an interface between variable and constant domains. Exposure of such hydrophobic residues to solvent increases the likelihood of aggregation.

[0055] In addition, the antibody mimics described herein have no disulfide bonds, which have been reported to retard or prevent proper folding of antibody fragme...

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Abstract

Disclosed herein are proteins that include a fibronectin type III domain having at least one randomized loop. Also disclosed herein are nucleic acids encoding such proteins and the use of such proteins in diagnostic methods and in methods for evolving novel compound-binding species and their ligands.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of the filing date of provisional application, U.S. Ser. No. 60 / 111,737, filed Dec. 10, 1998, and utility application, U.S. Ser. No. 09 / 456,693, filed Dec. 9, 1999.BACKGROUND OF THE INVENTION [0002] This invention relates to protein scaffolds useful, for example, for the generation of products having novel binding characteristics. [0003] Proteins having relatively defined three-dimensional structures, commonly referred to as protein scaffolds, may be used as reagents for the design of engineered products. These scaffolds typically contain one or more regions which are amenable to specific or random sequence variation, and such sequence randomization is often carried out to produce libraries of proteins from which desired products may be selected. One particular area in which such scaffolds are useful is the field of antibody design. [0004] A number of previous approaches to the manipulation of the mam...

Claims

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Application Information

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IPC IPC(8): C07H21/04C07K14/745C07K14/78G06Q50/00C07K16/24C12N15/10C12N15/62C12P21/06C12Q1/00C40B40/02G06Q10/00
CPCC07K14/78C07K16/241C07K2317/21C07K2318/20C40B40/02C12N15/1037C12N15/62C12Q1/00C07K2319/30
Inventor LIPOVSEK, DASAWAGNER, RICHARDKUIMELIS, ROBERT
Owner BRISTOL MYERS SQUIBB CO
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