Electrophoresis process using ionic liquids
a technology of ionic liquids and electrophoresis gels, which is applied in the direction of fluid pressure measurement, liquid/fluent solid measurement, peptides, etc., can solve the problems of organic solvents that are not compatible with the electrophoresis gel or the aqueous system used to form the gel, and the current electrophoresis system is limited
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example 1
[0070] This example demonstrates the formation of a non-aqueous electrophoresis gel.
[0071] A polyurethane prepolymer was obtained from Dow Chemical Co. under the trademark HYPOL G-50 and 1 part by volume (3.2 g) was combined with 6 parts by volume water (19.2 g). The components were mixed well and allowed to polymerize. The resulting gel was allowed to dry over several days in air at room temperature.
[0072] A first sample of the dried gel (0.5446 g) was covered with ethylene chloride in a closed container and allowed to stand overnight at room temperature. The resulting gel was clear, soft and pliable. The gel was weighed (6.18 g) which demonstrated an increase in weight of 11.3 fold.
example 2
[0073] A polyurethane electrophoresis gel was prepared in the absence of water in this example.
[0074] A 50% solution of HYPOL G-50 from Dow Chemical Co. was obtained in methylene chloride. A 50% v / v solution was prepared from 0.5 ml (0.45 g) of ethylenediamine and 0.5 ml of methylene chloride. 1.0 ml of the 50% solution of the prepolymer G-50 was mixed with 28.3 μl of the 50% solution of ethylenediamine. The resulting mixture gelled immediately.
example 3
[0075] The solution of the prepolymer G-50 of Example 2 was diluted with dry methylene chloride to obtain a 15% wt / vol concentration. The solution of the ethylenediamine was diluted with dry methylene chloride to obtain a 10% solution. 21 μl of the 10% ethylenediamine solution was combined with 1 ml of methylene chloride. Thereafter, 1 ml of the 15% solution of the prepolymer G-50 was added. The resulting mixture polymerized in about 1-2 minutes to produce a clear gel with no water and no bubbles.
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