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Human albumin animal models for drug evaluation, Toxicology and immunogenicity studies

a human albumin and animal model technology, applied in the field of human albumin animal model for drug evaluation, toxicology and immunogenicity studies, can solve the problem of extremely disappointing results when used in human trials

Inactive Publication Date: 2006-02-02
CARTER DANIEL C
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008] Accordingly, it is thus an object of the present invention to create animal models which supplement or replace the natural albumin sequence with a typical human serum albumin sequence and which thus will be far more effective in providing an accurate assessment of how drugs or other chemicals will react in humans.
[0009] It is another object of the present invention to create transgenic animals which contain and / or express human serum albumin and which will thus be far more useful in methods of drug evaluation, toxicology and immunogenicity and provide a far more accurate picture of how drugs, chemicals and vaccines will react in humans than would be possible through conventional testing of animals that do not express human albumin.
[0010] It is still further an object of the present invention to create a variety of transgenic animals from a variety of species which will contain and express human serum albumin and which will thus be far more useful in assessing the safety and efficacy of new drugs, vaccines, or other medicaments.
[0011] It is yet another object of the present invention to provide even further animal models for assessing the safety and efficacy of materials such as drugs or vaccines which are intended to be administered internally to humans, said models which are either natural mutants which do not express their own serum albumin or mammals which have had the gene for serum albumin knocked out, and such models can be injected with human serum albumin which is retained in these mammals and thus provides an animal model for assessing drugs or vaccines in humans more accurately than in animal models which do not contain or express human serum albumin.

Problems solved by technology

As a result, the literature is replete with examples of drugs which appeared to be extremely effective in animal testing only to show extremely disappointing results when used in human trials.

Method used

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  • Human albumin animal models for drug evaluation, Toxicology and immunogenicity studies

Examples

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example 1

Injection of Albumin into NAR Rats

[0035] In accordance with the present invention, a natural mutant rat which does not express or contain its own serum albumin, namely an NAR rat identified as ODNAR10, was utilized as an animal model in accordance with the present invention by injecting the animal with suitable amounts of human serum albumin. In this example, the NAR was given intravenous albumin injection / infusions by initially injecting relatively small amounts of HSA solution into the animal (1 mL). The amount of blood administered to this initial NAR rat was monitored over a 25 day period and this monitoring showed that a significant amount of human albumin had accumulated in the rat's system during this time, as shown by the chromatograph of FIG. 1, wherein column 10 represents the blood sample obtained from ODNAR10. By the end of the treatment (when the animal expired during anesthesia), ODNAR10 had received a total of 2.125 g HSA administered over a 25 day period (Table I)....

example 2

Transgenic NAR Rats

[0039] In accordance with the present invention, transgenic animal models were prepared which were transfected with the gene for human serum albumin. In this example, first generation NAR rat pups were sent to TOSK for injection of the HSA gene within their proprietary STEALTHGENE vector. Pups were sent to TOSK between the weights of 80 and 120 g, as per their instructions, due to their procedure being most amenable to this age and weight of rat. From the breeding program we have instigated for NAR rats, we have learned that females need to be on the order of 230 g before they conceive reliably. As of today, our heaviest females returned from TOSK have been transfected with the gene for human serum albumin and are 185.5 g and 190 g and growing at a rate of about 15 g every 3 to 4 days. In accordance with the invention, these animals are thus suitable as models for animal testing, and because they have been genetically transformed, such animals will be useful in ...

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Abstract

An animal model is provided which is genetically engineered to express human serum albumin, and such animals may be advantageously used in assessing drugs, vaccines or other therapeutic compounds that may be used in humans. In addition, an animal model is provided which does not manufacture its own albumin and which has been injected with human serum albumin. Through the use of these animal models, drugs and other chemicals can be more accurately assessed in physiological environments that reflect the conditions to be expected in humans, and such models will be useful in assessing new drugs and evaluating toxic substances for potential dangers as carcinogens, mutagens, etc. Other applications include evaluating immunological properties of various albumin-engineered proteins which might be administered to humans as therapeutics or vaccines, and research of disease states, such as genetic diseases, to provide further insight in treating these diseases.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a divisional application of U.S. Ser. No. 10 / 171,688, filed Jun. 17, 2002. This application also claims the benefit of U.S. Provisional Application Ser. No. 60 / 298,124, filed Jun. 15, 2001, incorporated herein by reference.FIELD OF THE INVENTION [0002] This invention relates in general to an animal model with serum attributes which have similar or identical properties and pharmokinetics with the human blood system in order to improve the assessment and development of new therapeutics and vaccines and to provide more reliable systems for identifying chemicals or drugs that may be carcinogenic or toxigenic to humans, and more specifically relates to transgenic animals and other animal models which contain and / or express human serum albumin and which thus can be used in methods of drug evaluation, toxicology and immunogenicity so as to provide a far more accurate picture of how drugs and chemicals will react in humans t...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01K67/027C07K14/765C12N15/85
CPCA01K67/0278A01K2207/15A01K2217/00C12N15/8509A01K2227/105A01K2267/03C07K14/765A01K2217/05A61P43/00
Inventor CARTER, DANIEL C.
Owner CARTER DANIEL C
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