Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement
a technology of applied in the field of plant extracts with antioxidant activity and anti-aging activity, and cosmetic compositions containing the extract for anti-oxidation, skin aging protection and wrinkle improvement, can solve the problems of low radical scavenging activity, and low individual oriented study on aging. , to achieve the effect of increasing the dpph scavenging activity, low radical scavenging activity, and high radical
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example 7
Activity to Protect Cells from Oxidative Stress Caused by UV Irradiation
Cell Protecting Effect from UV Irradiation (in Vitro)
[0115] UV irradiation induces DNA damage and DNA-protein connection by increasing active oxygen in cells. When HEK-N / F cells were irradiated by 35 mJ / cm2 of UVB, DNA chains in nucleus were cut. After uniting the broken chains to ethidium homodimer (Et2), a DNA chain intercalating fluorescent dye, extent of fluorescence was measured to quantify the amount of DNA damage. Particularly, HEK-N / F cells were cultured on serum-free KGM medium (Clonetics), which were inoculated into wells of a 24-well plate by 0.5-4×104 cells / 2 cm2. Then, the cells were cultured for 18 hours, after which a sample was treated thereto for 2 hours. After being washed with PBS, the cells were irradiated by UV using a UV transilluminator (Spectronics UV transilluminator EBF-260, the maximal wavelength, 312 nm; a half-peak intensity range, 297-328 nm). The cells exposed on UV were further...
example 8
Prolongation of Life Span of a Cell
[0122] HEK-N / F cells were obtained from foreskin of a 5 newborn baby after its fibroblasts, another major component of skin, were treated with uridine bromide. Then, the cells were cultured in DMEM medium supplemented with 10% FBS. HEK-N / F cells were diluted consecutively at the ratio of 1:4 during the culture. Before being treated with samples, the cells were grown up to 3 PDL (population doubling level). During the culture, each sample was administered by 3 g / ml. Medium was replaced every three days by fresh medium supplemented with a culture solution containing the equal concentration of the sample. After culturing, an extract of Cercis chinensis of the present invention, a compound represented by (piceatannol) and a compound represented by (myricetin) were treated, followed by investigation of the cell division.
[0123] As a result, an extract of Cercis chinensis and its active ingredients were all confirmed to have an effect of prolonging li...
example 9
Prolongation of Life Span of a Cell and Extension of a Telomere
[0124] In the above Example 8, an extract of Cercis chinensis and active ingredients separated from the same were confirmed to have an effect of prolongation of life span of a cell. Thus, in this example, relation between the effect of prolongation of life span and the length of a telomere was investigated. Particularly, genomic DNA was extracted from each cell of different ages by using a nucleic acid extraction kit (IsoQuick Nucleic Acid Extraction kit, ORCA Research Inc.), which was stored at 4° C. after being dissolved in Tris-EDTA solution (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). 2 μl of 10×H buffer solution (TaKaRa), 2 μl of the extracted genomic DNA solution and distilled water were all mixed in a 1.5 ml tube, making final volume 19 μl. Then, 1 M of restriction enzyme Hinf I (6 U / l, TaKaRa) was added thereto. The mixture was reacted at 37° C. for 3-4 hours, followed by electrophoresis. For the electrophoresis, agaros...
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