Drug targets in Candida albicans
a technology of candida albicans and drug targets, applied in the field of drug targets in candida albicans, can solve the problems of increasing the frequency of opportunistic infections in immunocompromised hosts, the general limitation of the use of drugs, and the difficulty of detecting and treating candida albicans
Inactive Publication Date: 2007-01-11
CONTRERAS ROLAND HENRI +6
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Benefits of technology
This approach allows for the selective identification and development of compounds that can effectively treat Candida albicans infections with reduced toxicity and improved understanding of their mechanism of action, providing a more targeted therapeutic strategy.
Problems solved by technology
Opportunistic infections in immunocompromised hosts represent an increasingly common cause of mortality and morbidity.
Such fungal infections are thus problematical in, for example, AIDS populations in addition to normal healthy women where Candida albicans yeasts represent the most common cause of vulvovaginitis.
Although compounds do exist for treating such disorders, such as, amphotericin, these drugs are generally limited in their treatment because of their toxicity and side effects.
These tests are cumbersome and provide no information about a compounds mechanism of action.
Method used
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example 1
[0068] Identification of Novel Drug Targets in C. albicans by Anti-Sense and Disruptive Integration
[0069] The principle of the approach is based on the fact that when a particular C. albicans mRNA is inhibited by producing the complementary anti-sense RNA, the corresponding protein will decrease. If this protein is critical for growth or survival, the cell producing the anti-sense RNA will grow more slowly or will die.
[0070] Since anti-sense inhibition occurs at mRNA level, the gene copy number is irrelevant, thus allowing applications of the strategy even in diploid organisms.
[0071] Anti-sense RNA is endogenously produced from an integrative or episomal plasmid with an inducible promoter; induction of the promoter leads to the production of an RNA encoded by the insert of the plasmid. This insert will differ from one plasmid to another in the library. The inserts will be derived from genomic DNA fragments or from cDNA to cover—to the extent possible—the entire genome.
[0072] The...
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Abstract
The present invention is concerned with the identification of genes or functional fragments thereof from Candida albicans which are critical for growth and cell division and which genes may be used as selective drug targets to treat Candida albicans associated infections. Novel nucleic acid sequences from Candida albicans are also provided and which encode the polypeptides which are critical for growth of Candida albicans. Methods for the identification of anti-fungal compounds which inhibit fungal or yeast growth are also contemplated.
Description
CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a division of U.S. Ser. No. 09 / 857,372, filed Jun. 4, 2001, which was a National Stage application under 35 U.S.C. § 371 of International Application No. PCT / EP99 / 09833 filed 6 Dec. 1999, which claims priority from EP98204122.0, filed 4 Dec. 1998, the contents of which are hereby incorporated by reference.FIELD OF THE INVENTION [0002] The present invention is concerned with the identification of genes or functional fragments thereof from Candida albicans which are critical for growth and cell division and which genes may be used as selective drug targets to treat Candida albicans associated infections. Novel nucleic acid sequences from Candida albicans are also provided and which encode the polypeptides, which are critical for growth of Candida albicans. BACKGROUND OF THE INVENTION [0003] Opportunistic infections in immunocompromised hosts represent an increasingly common cause of mortality and morbidity. Candida spe...
Claims
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IPC IPC(8): A61K38/16C07H21/04C12N15/74C12N1/18C07K14/40G01N33/50A61K31/7088A61K38/00A61K45/00A61P15/02A61P31/10C07K16/14C12N1/15C12N1/19C12N1/21C12N5/10C12N15/09C12N15/31C12Q1/02C12Q1/68C12R1/725G01N33/15
CPCC07K14/40A61K38/00A61P15/02A61P31/10
Inventor CONTRERAS, ROLAND HENRINELISSEN, BART JOZEF MARIADE BACKER, MARIANNE DENISELUYTEN, WALTER HERMAN MARIA LOUISVIAENE, JASMINE ELZALOGGHE, MARC GEORGEVIALARD, JORGE EDUARDO
Owner CONTRERAS ROLAND HENRI