Protease susceptibility II

a tropoelastin and susceptibility technology, applied in the field of tropoelastin susceptibility ii, can solve the problems of inadequate or faulty elastin fiber repair at the site of injury, the soluble precursor of elastin is far more susceptible to proteolysis, and the elastin molecule is not shown in the study

Inactive Publication Date: 2008-03-06
APTALIS PHARMA
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Problems solved by technology

However, tropoelastin, the soluble precursor of elastin, is far more vulnerable to proteolysis.
However, proteolysis could also result in inadequate or faulty elastin fiber repair at the site of injury.
However, none of these studies has provided indication of where the tropoelastin molecule is cut by proteases.

Method used

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  • Protease susceptibility II
  • Protease susceptibility II
  • Protease susceptibility II

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Materials and Methods

Reagents

[0140] Hirudin, PMSF, human thrombin, human plasma kallikrein, human plasmin and human leukocyte elastase (HLE) were obtained from Sigma. Bovine trypsin and Pefabloc SC were from Boehringer-Mannheim and Pefabloc PK was from Pentapharm, Switzerland. Gelatinase A (72 kDa gelatinase) and gelatinase B (92 kDa gelatinase) were obtained from Boehringer Mannheim Roche Diagnostics.

[0141] SHEL was obtained by the method described in WO94 / 14958.

[0142] SHELδ26A can be derived from SHEL by removing the synthetic coding sequence corresponding to exon 26A. A comparison of the sequence of SHEL with that of SHELδ26A is provided at FIG. 3. Its protein product is apparently identical to a naturally made human splice form of tropoelastin.

[0143] The Transformer Mutagenesis Kit (Clontech USA) was used with pSHELF (described in WO94 / 14958) in accordance with the supplied protocol to remove DNA corresponding to exon 26A. The sequence of the mutagenic primer used (manufac...

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Abstract

The manipulation of the amino acid sequence of tropoelastin, particularly human tropoelastin, to modify its protease susceptibility is described. The modified tropoelastins include tropoelastin derivatives having modified protease susceptibility, peptidomimetic molecules which contain amino acid sequences which correspond to or incorporate the protease susceptible sequences of tropoelastin. Uses of these tropoelastin derivatives and peptidomimetic molecules are provided. Also provided are nucleic acid molecules and genetic constructs encoding the amino acid sequences of the derivatives and peptidomimetic molecules of the invention.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 09 / 743,818, filed Apr. 26, 2001, which is a 371 of PCT / AU99 / 00580, filed Jul. 19, 1999.BACKGROUND OF THE INVENTION [0002] The present invention relates to: manipulation of the amino acid sequence of tropoelastin, particularly human tropoelastin, to modify its protease susceptibility; to tropoelastin derivatives having modified protease susceptibility; to peptidomimetic molecules which contain amino acid sequences which correspond to or incorporate the protease susceptible sequences of tropoelastin; and to uses of the tropoelastin derivatives and peptidomimetic molecules. [0003] The invention also relates to nucleic acid molecules and genetic constructs encoding the amino acid sequences of the derivatives and peptidomimetic molecules of the invention. [0004] The insoluble cross-linked elastin molecule is highly resistant to proteolytic degradation by many proteases. H...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/00A61P43/00C07K14/00C07K7/00C12N15/09A61K38/17A61L27/00C07H21/04C07K7/02C07K7/04C07K14/78C07K19/00C12N1/15C12N1/19C12N1/21C12N5/10C12N15/12C12P21/02
CPCA61K38/00C07K14/78C07K7/02A61P43/00
Inventor WEISS, ANTHONY STEVEN
Owner APTALIS PHARMA
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