Plant Stress Tolerance from Modified Ap2 Transcription Factors

Inactive Publication Date: 2008-09-18
MENDEL BIOTECHNOLOGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Abiotic stresses, including freezing temperatures, drought and high salinity, and biotic stresses, including disease, greatly limit the geographical locations where crops can be

Method used

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  • Plant Stress Tolerance from Modified Ap2 Transcription Factors
  • Plant Stress Tolerance from Modified Ap2 Transcription Factors
  • Plant Stress Tolerance from Modified Ap2 Transcription Factors

Examples

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examples

[0196]The invention, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention and are not intended to limit the invention. It will be recognized by one of skill in the art that a transcription factor that is associated with a particular first trait may also be associated with at least one other, unrelated and / or inherent second trait that was not predicted by the first trait.

example i

Full Length Gene Identification and Cloning

[0197]Putative transcription factor sequences (genomic or ESTs) related to known transcription factors were identified in the Arabidopsis thaliana GenBank database using the tblastn sequence analysis program using default parameters and a P-value cutoff threshold of −4 or −5 or lower, depending on the length of the query sequence. Putative transcription factor sequence hits were then screened to identify those containing particular sequence strings. If the sequence hits contained such sequence strings, the sequences were confirmed as transcription factors.

[0198]Alternatively, Arabidopsis thaliana cDNA libraries derived from different tissues or treatments, or genomic libraries were screened to identify novel members of a transcription family using a low stringency hybridization approach. Probes were synthesized using gene specific primers in a standard PCR reaction (annealing temperature 60° C.) and labeled with 32P dCTP using the High Prim...

example ii

In Vitro Mutagenesis

[0201]One method that may be used to create a transgenic plant overexpressing a modified transcription factor may be with the use of physical or chemical mutagenizing agents that may be used directly on isolated DNA. For example, an isolated AP2 polynucleotide sequence may be subjected to UV irradiation, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, or nucleotide analogues. When such agents are used, the mutagenesis is typically performed by incubating the DNA sequence encoding a transcription factor to be mutagenized in the presence of the mutagenizing agent of choice under suitable conditions well known in the art. The DNA may then be incorporated into a vector and transformed into a plant cell, which may then be regenerated into a plant. It may be desirable to amplify the mutated DNA (for example, using PCR) prior to insertion into the vector.

[02...

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Abstract

The invention relates to modified plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including increased abiotic or biotic stress tolerance, as compared to wild-type or control plants. The modifications to the plant transcription factor sequences are responsible for producing fewer and less severe adverse morphological and developmental characteristics in plants overexpressing these sequences than would be caused by overexpressing the sequences without the modifications.

Description

FIELD OF THE INVENTION[0001]The present invention relates to compositions and methods for producing plants with improved stress tolerance.BACKGROUND OF THE INVENTION[0002]Abiotic stresses, including freezing temperatures, drought and high salinity, and biotic stresses, including disease, greatly limit the geographical locations where crops can be grown and cause significant losses in productivity on an annual basis. Many of the transcription factor genes that have been shown to confer abiotic or biotic stress tolerance in plants are AP2 family genes. However, overexpression of AP2 transcription factors tend to create plants that are smaller than wild-type. It is thus likely that there are conserved residues / motifs in all of these proteins that cause such effects. Ideally, AP2 polypeptides may be found or created that lack these conserved residues / motifs and can yet confer increased abiotic stress tolerance (e.g., freezing, chilling or drought tolerance) or biotic stress (disease tol...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8282C12N15/8273
Inventor LIBBY, JEFFREY M.RATCLIFFE, OLIVER J.GUTTERSON, NEAL ICREELMAN, ROBERT A.
Owner MENDEL BIOTECHNOLOGY INC
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