Method for Preparation of Extract from Angelica Gigas Nakai and Composition Containing the Extract

a technology of extract and composition, applied in the field of preparation of extract of angelica gigas nakai and composition containing the extract, can solve the problems of severe and undesirable process, and achieve the effects of enhancing performance, preventing hydrolysis of natural substances, and excellent antioxidant and analgesic effects

Inactive Publication Date: 2011-01-06
AZI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Therefore, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a process for preparation of an extract from Angelica gigas Nakai with improved performances wherein the extract contains decursin, decursinol angelate and decursinol in relative compositional ratios of 50 to 200:1 in terms of (decursin+decursinol angelate) to decursinol and, in addition, a composition including the extract.
[0017]Accordingly, the present invention is directed to development of a process for preparation of an extract containing functional components with specified compositional ratios thereof under mild conditions instead of severe conditions described above, as well as a composition including the extract prepared by the above process. In addition, another object of the present invention is to provide a composition with superior analgesic effects over those of pure decursinol.
[0019]As described above, the present invention is characterized in that a favorably mild process is conducted to produce Angelica gigas Nakai extract in place of a conventional strong alkaline treatment, so as to obtain a natural extract-like product while considerably enhancing performance thereof. Therefore, the present invention has merits of preventing hydrolysis of natural substances due to a severe alkaline treatment and producing an extract composition with excellent antioxidant and analgesic effects.

Problems solved by technology

However, this is a very severe and undesirable process for the extract and hydrolysis of numerous natural substances occurring under strong alkaline conditions is duly expected.

Method used

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  • Method for Preparation of Extract from Angelica Gigas Nakai and Composition Containing the Extract
  • Method for Preparation of Extract from Angelica Gigas Nakai and Composition Containing the Extract
  • Method for Preparation of Extract from Angelica Gigas Nakai and Composition Containing the Extract

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of an Extract from Angelica gigas Nakai Using Alcohol

[0048]10 kg of air dried Angelica gigas Nakai herb was purchased at a marketplace named “Kyung-dong market,” in Korea. 1 kg of the herb was used. 5 L of 93% (v / v) ethanol was prepared by diluting alcohol, followed by adding 15 wt. % of sodium hydroxide solution thereto so as to prepare an ethanol solvent with pH 8.5. The herb was added to the prepared solvent and subjected to extraction at 65□. Sampling the extract at 15-minute intervals, the sample underwent pH measurement using a pH measuring tape, followed by adjusting the pH value to 8.0. 4 hours after the extraction, the pH adjustment was terminated and additional extraction was conducted for 1 hour. After the extraction, a filtrate was separated and concentrated to a volume of 3 L. Settling the concentrate at 10□ for 10 hours, a settled precipitate was filtered and removed to yield a solution which in turn was again subjected to concentration. Adding 100 g of mic...

example 2

Preparation of an Extract from Angelica gigas Nakai Using Water

[0050]Adding 100 g of water to Angelica gigas Nakai herb prepared as described in Example 1, the mixture was subjected to extraction for 3 hours. The obtained concentrate was dried and 21 g of the dried product was added to 21 g of microcrystalline cellulose. Adding a small amount of water re-dissolved the mixture. The re-dissolved solution was dried to yield 41 g of powder as a final product, which is Angelica gigas Nakai extract powder.

[0051]The extract was analyzed by gas chromatography and the results show that a relative content ratio of (decursin+decursinol angelate) to decursinol was 825:1 and a content of decursinol was 0.0083 wt.%.

[0052]From the above results in Examples 1 and 2, it is found that Angelica gigas Nakai extract obtained using water in Example 2 has decursinol content 18 times that of the extract obtained using alcohol in Example 1.

example 3

Comparison of Antioxidant Effects

[0053]Antioxidant effects were compared between both extract powders obtained in Examples 1 and 2. Using DPPH analyzed and quantified EDA to determine the antioxidant effects of the extract powder. IC50 means a concentration required for scavenging 50% of radicals and it is determined that the antioxidant effects are enhanced as IC50 value is lowered. It was demonstrated that the extract prepared according to the present invention has antioxidant effects 10 times better than those of an extract obtained using water as a control.

TABLE 1Comparison of antioxidant effects of Angelica gigas Nakai extractsIC50 (mg / L)Vitamin E15Angelica gigas Nakai230extract obtained usingwaterExtract of the present22invention

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PUM

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Abstract

Disclosed is Angelica gigas Nakai extract powder prepared by a process including: (a) preparing 85-100% (v / v) pure ethanol and adjusting a pH value of the ethanol to pH 7-9 with an alkaline solution; (b) adding 3-10× of the prepared ethanol to the total weight of Angelica gigas Nakai herb, which is naturally grown or harvested; (c) conducting extraction of the same at 30-75° C. for 3-8 hrs; (d) measuring a pH value of the treated mixture at 15 m intervals and adjusting the same to pH 7-9 with the alkaline solution during the extraction, while stopping the pH control at 1 hr prior to completion of the extraction; (e) filtering and concentrating the obtained extract to 3× the net weight of Angelica gigas Nakai; (f) cooling and settling the treated mixture at 10-30° C. for 1-24 hrs to remove a settled precipitate; (g) adding an excipient such as microcrystalline cellulose, dextrin, etc. to a concentrate remaining after removal of the precipitate; and (g) drying and pulverizing the resulting product.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to a method for preparation of an extract of Angelica gigas Nakai and a composition containing the extract prepared by the same, more particularly, to a method for preparation of an ethanol extract of Angelica gigas Nakai, which contains decursin, decursinol, and decursinol angelate in a relative ratio of 50 to 200:1 in terms of (decursin+decursinol angelate) to decursinol known as active ingredients, so as to accomplish considerably improved antioxidant and analgesic effects.[0003]2. Description of the Related Art[0004]Angelica gigas Nakai is well known as a nontoxic medical herb and is widely used as a folk medicine. Oriental herbal medicine is typically used in an extract form prepared by hot water extraction and medical efficacies thereof have been studied and disclosed in prior documents including, for example: S. M. Kim et al., Journal of the Korean Society of Food Science and Nutriti...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/23A61P39/06
CPCG01N33/57415G01N33/57423G01N33/57426G01N33/57438H01J49/0027G01N33/84C12Q1/04G01N33/49G01N33/57484G01N33/57446A61P39/06G01N33/48G01N33/53G01N33/574
Inventor PARK, SANG JAECHOI, WON CHEOLKWON, SUNG PIL
Owner AZI
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