Preventive and/or ameliorative agent for diseases, stamina enhancement agent, Anti-fatigue agent, and pharmaceutical and food and drink using them
a technology of anti-fatigue agent and prevention and/or amelioration agent, which is applied in the direction of immunological disorders, bacteria based processes, metabolism disorders, etc., can solve the problems of difficult long-term use and adverse effects of using, and achieve superior efficacy and safety, improve performance, and improve the effect of health conditions of non-healthy subjects
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example 1
[0155]In the present example, effects of purple non-sulfur bacteria on inflammatory bowel diseases were histologically evaluated using model mice with colitis induced by dextran sulfate sodium (DSS).
[0156](Culture of Bacterial Cell)
[0157]First, 0.4% by weight sodium acetate and 5% by weight sucrose were added to a basal medium having the composition shown in Table 4, which then was adjusted to pH 7.0. Thus, a medium for bacterial cell was prepared. The medium for bacterial cell was placed in a sealed and illuminated culture vessel. Then a 20% by volume bacterial suspension of Rhodobacter azotoformans BP0899 (Accession No. NITE BP-644) with a bacterial concentration of 1×106 cells / cm3 was added to the medium, which then was subjected to spinner culture at 30° C. for 7 days. Bacterial cells were collected from a culture solution obtained after the spinner culture using a continuous centrifugal separator (sharpless type). Then the bacterial cells were adjusted to 1×1011 cells / cm3 and c...
example 2
[0165]Water and feed were administered into five 5-week-old female ICR mice (purchased from Japan SLC, Inc.) in the same manner as in Example 1 except that the period of ingesting the water containing no DSS and the feed containing bacterial cells was 4 weeks. Then histological evaluations of ulcer formation and a regenerated epithelium were conducted.
example 3
[0172]In the present example, effects of the administration amount of purple non-sulfur bacteria on inflammatory bowel diseases were histologically evaluated using model mice with colitis induced by dextran sulfate sodium (DSS).
[0173](Preparation of Bacterial Cell Powder)
[0174]In the present example, a bacterial cell powder was prepared in the same manner as in Example 1.
[0175](Oral Administration Test)
[0176]In an oral administration test, 3 groups (2 to 4 mice for each group) of 5-week-old female ICR mice (purchased from Japan SLC, Inc.) were used. For induction of colitis in the mice, a DSS-containing water containing 5 w / v % of dextran sulfate sodium (having the molecular weight from 36000 to 50000, manufactured by ICN biomedicals, Inc.) was prepared. The mice in each of the groups ingested the DSS-containing water ad libitum, and the predetermined amounts (1, 6, and 10 mg / kg, p.o.) of the same bacterial cell powder as in Example 1 were administered into the respective mice once ...
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