Tumor treatment
a tumor and tumor technology, applied in the field of tumor treatment, can solve the problem that the inhibition of thymidylate synthase alone is likely approaching the limit of colorectal cancer efficacy, and achieves the effect of improving the effect of thymidylate synthase and the effect of reducing the risk of cancer
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Treatment of Human Colorectal Tumor Xenografts with Irinotecan (CPT-11) Activates Genes Normally Expressed by Squamous Cell Epithelium
[0168]This study was designed to identify gene transcripts acutely expressed by human colorectal adenocarcinomas following in vivo exposure to the standard care chemotherapeutic irinotecan. Colo205 and DLD-1 xenografts were used as p53 wild-type (wt) and p53 mutant tumor models, respectively, and gene expression by normal murine colon tissue resected from the same animals was also analyzed. The expression levels of numerous transcripts were reproducibly altered by drug treatment of the tumors, including, but not limited to, the genes normally expressed by squamous cell epithelium.
[0169]Materials and Methods
[0170]Cell lines—Colo205, HCT116, HT29 (ATCC Nos. CCL222, CCL221, CCL247, HTB38, respectively) are human colorectal adenocarcinoma cell lines. 293 is a human immortalized embryonic kidney cell line (ATCC CRL1573). PC-3 is a human prostate adenocarci...
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