I-crei meganuclease variants with modified specificity, method of preparation and uses thereof

a meganuclease and specificity technology, applied in the field of i-crei meganuclease variants with modified specificity, method of preparation and use thereof, can solve the problems of low efficiency, limited technology, and inability to have a natural meganuclease cleaving a gene of interes
US20120258537A1Active Publication Date: 2012-10-11CELLECTIS SA
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
CELLECTIS SA
Publication Date
2012-10-11

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Abstract

Method of preparing I-CreI meganuclease variants having a modified cleavage specificity, variants obtainable by said method and their applications either for cleaving new DNA target or for genetic engineering and genome engineering for non-therapeutic purposes. Nucleic acids encoding said variants, expression cassettes comprising said nucleic acids, vectors comprising said expression cassettes, cells or organisms, plants or animals except humans, transformed by said vectors.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application is a continuation of U.S. Ser. No. 12 / 859,905, filed Aug. 20, 2010, which is a continuation of U.S. Ser. No. 11 / 908,798, filed on Sep. 17, 2007, which is a 35 U.S.C. §371 National Stage patent application of PCT / IB2006 / 001203, filed on Mar. 15, 2006, which claims priority under 35 U.S.C. §120 and 35 U.S.C. §365(c) to International patent application PCT / IB2005 / 003083, filed on Sep. 19, 2005, and also claims priority under 35 U.S.C. §120 and 35 U.S.C. §365(c) to International patent application PCT / IB2005 / 000981, filed Mar. 15, 2005.BACKGROUND OF THE INVENTION

[0002] The present invention relates to a method of preparing I-CreI meganuclease variants having a modified cleavage specificity. The invention relates also to the I-CreI meganuclease variants obtainable by said method and to their applications either for cleaving new DNA target or for genetic engineering and genome engineering for non-therapeutic purposes.[000...

Claims

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