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Thyroxine immunoassay using fluorescent particles

a technology of fluorescent particles and immunoassays, applied in the field of base plates and methods for immunoassays of thyroxine using fluorescent particles, to achieve the effect of reducing the signal change rate caused by temperature change in the immunoassay of thyroxin

Active Publication Date: 2013-03-28
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a method for measuring thyroxine in a more accurate way by using a base plate onto which a certain amount of thyroxine or a derivative thereof is immobilized. This base plate is then brought into contact with anti-thyroxine antibody-labeled fluorescent particles and thyroxine as a test substance. This results in the thyroxine on the base plate and the test substance competitively binding to the anti-thyroxine antibody-labeled fluorescent particles, which can be measured based on the fluorescence. This method can decrease the signal change rate caused by temperature change and provides a more reliable measure of thyroxine levels.

Problems solved by technology

However, since enzymatic reactions exhibit high temperature dependency, to carry out strict temperature management at the time of reaction is necessary.

Method used

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  • Thyroxine immunoassay using fluorescent particles

Examples

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Effect test

example 1

1. Preparation of Powder of Thyroxine-Bovine Serum Albumin (T4-BSA) Conjugate-1

[0060]1-1. Synthesis of T4 Derivative

[0061]A T4 derivative 1 and a T4 derivative 2 were synthesized as illustrated in Scheme 1 below.

[0062]Thyroxine (T4, manufactured by Sigma-Aldrich Co.) was prepared, and a T4 derivative 1 was synthesized according to the synthesis method described in U.S. Pat. No. 4,040,907B. 270 mg of the T4 derivative 1 thus obtained was added to dimethylformamide (DMF; manufactured by Wako Pure Chemical Industries, Ltd.), and the derivative was dissolved while being stirred at room temperature. Thereafter, 285 mg of WSC (manufactured by Dojindo Laboratories Co. Ltd.) and 174 mg of NHS (manufactured by Wako Pure Chemical Industries, Ltd.) were added to the solution, and the mixture was stirred for 2 hours at room temperature. Thus, a T4 derivative 2 was synthesized.

[0063]1-2. Solution of T4-BSA Conjugate-1

[0064]Next, a conjugate (T4-BSA) of the T4 derivative 2 obtained in section 1-1...

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Abstract

Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to a base plate and a method for immunoassay of thyroxine using fluorescent particles.[0003]2. Description of the Related Art[0004]Fluorescence detection methods have been hitherto used in a large variety of fields, as analytic methods that are highly sensitive and simple and are capable of quantitatively determining proteins, enzymes, inorganic compounds and the like. These fluorescence detection methods are methods by which a sample which is suspected to contain a substance to be detected (test substance) that is excited by light having a specific wavelength and emits fluorescence, is irradiated with excitation light having the specific wavelength, fluorescence is detected at that time, and thereby the presence of the test substance is verified. Furthermore, in the case where the substance is not a fluorescent material, a method of bringing a substance which specifically binds to a test s...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/78G01N21/64
CPCG01N33/54306G01N33/78G01N33/585G01N33/54373
Inventor KASAGI, NORIYUKIKATADA, JUNICHIMATSUNO, TADAHIRO
Owner FUJIFILM CORP
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