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Colon and pancreas cancer specific antigens and antibodies

a cancer and specific antigen technology, applied in the field of tumor molecular biology, can solve the problems of affecting the patient's health, affecting the detection and treatment of cancer, and affecting the success of cancer diagnosis and treatment, so as to promote tumor regression and slow the growth of tumors

Inactive Publication Date: 2013-07-25
PRECISION BIOLOGICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides isolated polypeptides that specifically bind to the NPC-1, 16C3, and 31.1 epitopes, which are tumor-specific antigens. These polypeptides may be used for the development of antibodies that can be used for diagnosis and treatment of tumors. The invention also provides fusion proteins and conjugates containing the tumor-specific antigen and a detectable label or therapeutic agent for use in diagnosis and treatment of tumors.

Problems solved by technology

In later stages of cancer, the tumor cells proliferate at an unusually high rate resulting in uncontrolled growth that threatens the health of the patient known as malignant tumors (or in situ cancer).
National Cancer Institute (2010) “What You Need to Know about Cancer of the Pancreas.” While there have been many advancements in cancer detection and therapy over the last 2 decades, nonetheless the current options for early detection and treatment of cancer are limited and there exists a great need for new methods and materials that provide for the detection and treatment of cancer, especially colorectal and pancreatic cancer.
The pursuit for monoclonal antibodies for cancer therapy and diagnostics has been hampered, at least in part, by the difficulty in characterizing tumor-specific epitopes.

Method used

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  • Colon and pancreas cancer specific antigens and antibodies
  • Colon and pancreas cancer specific antigens and antibodies
  • Colon and pancreas cancer specific antigens and antibodies

Examples

Experimental program
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example 1

Identification of the NPC-1 Antigen

[0360]The NPC-1 antibody was generated in mice immunized with the so-called “Hollinshead colon cancer vaccine”. Hollinshead, et al. (1985) Cancer 56: 480-489. The NPC-1 antibody and the chimeric form, NPC-1C, are described in U.S. Pat. Nos. 7,314,622 and 7,763,720. Several protein purifications were prepared using both mouse NPC-1 and recombinant, chimeric NPC-1C antibodies. Tumor cell lines including LS174T and HT-29 (human colorectal tumor), CFPAC-1 (human pancreatic tumor), colon cancer patient tumor specimen, and the Hollinshead colon cancer vaccines served as tumor antigen sources for protein extracts. The NPC-1 antigen is secreted into the medium of the human tumor cell lines, and the antigen was purified from tumor cell supernatant of cells grown in the absence of serum for 5 to 7 days. NPC-1 antibody was coupled to resins for the antigen purification, including magnetic beads, for simple adsorption, washing, and elution from the beads. Prot...

example 2

NPC-1 Antigen Knockdown

[0365]A small inhibitory RNA (siRNA) target sequence designed against a region of MUC5AC was used in cells known to express the NPC-1 antigen. Several siRNA oligonucleotides were designed based upon MUC5AC sequences. The sequences of the human MUC5AC siRNA oligonucleotides are shown in Table 7:

TABLE 7Sequence of MUC5AC siRNA oligonucleotidesOligonucleotideStrandSequencesiRNA ID #: s9074SenseAGAUGUGCCUCAACUACGAtt (SEQ ID NO: 120)Anti-SenseUCGUAGUUGAGGCACAUCUtg (SEQ ID NO: 121)siRNA ID #: s9075SenseGCUCUGGAACGUGAGCAUAtt (SEQ ID NO: 122)Anti-SenseUAUGCUCACGUUCCAGAGCcg (SEQ ID NO: 123)siRNA ID #: s9076SenseGCGUGCUCGUCGACAACUAtt (SEQ ID NO: 124)Anti-SenseUAGUUGUCGACGAGCACGCgg (SEQ ID NO: 125)

[0366]The siRNAs were transfected into LS174 and CFPAC-1 tumor cells, as well as A549 cells. A549 is a human lung adenocarcinoma cell line that expresses MUC5AC (as shown by PCR and detection using commercially available antibodies against MUC5AC), but not NPC-1 antigen. The MU...

example 3

NPC-1 Epitope Mapping

[0368]The data by western blots of trypsin-digested MUC5AC indicated that there may be several NPC-1 antibody binding sites on each molecule of MUC5AC, suggesting that a binding region might be present in each of the tandem repeat units located in the central region of the molecule. Therefore, a recombinant expression plasmid was designed and constructed that encoded residues upstream of tandem repeat units and two tandem repeat units (“MUC5AC-long” SEQ ID NO: 3). The MUC5AC long peptide corresponds to amino acid residues 2636 to 2942 of the MUC5AC protein (SEQ ID NO: 1). A second expression plasmid was designed and constructed that encoded primarily a short domain which connects to the central repetitive region and only a portion of the tandem repeat residues (“MUC5AC-short” SEQ ID NO: 5). The MUC5AC short peptide corresponds to amino acid residues 2636 to 2763 of the MUC5AC protein (SEQ ID NO: 1 and FIG. 1). These smaller fragments of the large MUC5AC molecule...

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Abstract

This invention relates to NPC-1 antigen on the MUC5AC protein and 16C3 antigen on CEACAM5 and CEACAM6 proteins, and 31.1 epitope on the A33 protein are differentially expressed in cancers including, lung cancer, ovarian cancer, pancreas cancer, breast cancer, and colon cancer, and diagnostic and therapeutic usages. Further, NPC-1, 16C3, and / or 31.1 epitope specific antibodies and diagnostic and therapeutic methods of use.

Description

CROSS-REFERENCE TO RELATED PATENT APPLICATIONS[0001]This International Patent Application claims priority to U.S. Provisional Patent Application No. 61 / 357,165, filed Jun. 22, 2010; U.S. Provisional Patent Application No. 61 / 359,440, filed Jun. 29, 2010; U.S. Provisional Patent Application No. 61 / 385,587, filed Sep. 23, 2010; U.S. Provisional Patent Application No. 61 / 407,112, filed Oct. 27, 2010; U.S. Provisional Patent Application No. 61 / 435,163, filed Jan. 21, 2011; U.S. Provisional Patent Application No. 61 / 435,176, filed Jan. 21, 2011; and U.S. Provisional Patent Application No. 61 / 467,896, filed Mar. 25, 2011, the disclosures of each of which are herein incorporated by reference.BACKGROUND OF THE INVENTIONMolecular Biology of Cancer[0002]Cancer is caused by a malfunction in the growth control systems of a cell. Cells control their growth via combination of proliferation inhibition by tumor suppressor genes (e.g., Retinoblastoma protein (pRb), p53) and proliferation activation ...

Claims

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Application Information

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IPC IPC(8): C07K14/705C07K7/08
CPCA61K51/1063C07K14/705C07K7/08C07K16/2803A61P35/00A61P37/04
Inventor DU, XIULIANLUKA, JANOSSTAFFORD, LEWIS JOESEMENUK, MARKWANG, XUE-PINGKANTOR, JUDITHBRISTOL, ANDREW J.
Owner PRECISION BIOLOGICS INC
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