Unlock instant, AI-driven research and patent intelligence for your innovation.

Probiotic Strains for Treating and/or Preventing Diarrhea

a technology of probiotic strains and diarrhea, applied in the field of probiotic strains, can solve the problems of diarrhea, more than 20 liters of fluid per day, and saturated absorption capacity of the stomach,

Inactive Publication Date: 2016-01-07
INST NAT DE RECH POUR LAGRICULTURE LALIMENTATION & LENVIRONNEMENT +2
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is based on the discovery that certain probiotic strains can directly act on diarrhea by inhibiting the absorption of water in the colon. This is surprising because traditional probiotic strains were thought to only inhibit the absorption of water by binding to transporters, which can lead to the inhibition of their functioning. The invention provides a method for selecting probiotic strains that can treat and prevent diarrhea by measuring the effect of the strain on the expression of transporters involved in water absorption. The probiotic strains can be used as a treatment or prevention for diarrhea of infectious or non-infectious origin. The invention also relates to a composition containing the probiotic strain for treating and preventing diarrhea. The strain used in the invention is Bacillus subtilis CU1 deposited at the CNCM on Oct. 25, 2001.

Problems solved by technology

However, above 6 liters of water per day, its capacity for absorption is saturated, and diarrhea develops.
Diarrhea is a common problem (worldwide, about two billion cases each year) which is characterized by stools of liquid or soft consistency, more bulky and frequent than usual (more than 3 bowel movements per day).
In extreme cases, more than 20 liters of fluid can be lost per day.
Its commonest cause is ingestion of contaminated water or food; in that case it lasts one or two days without requiring treatment.
However, diarrhea itself can cause dehydration that can prove fatal, especially in an infant, where a weight loss exceeding 10% is a hospital emergency.
Dehydration caused by diarrhea occurs when the losses of fluid are not compensated.
However, slowing of the transit time can be problematic in severe infections of the Salmonella, Shigella or Clostridium difficile type, since the pathogenic bacterium remains in the intestine for a long time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probiotic Strains for Treating and/or Preventing Diarrhea
  • Probiotic Strains for Treating and/or Preventing Diarrhea
  • Probiotic Strains for Treating and/or Preventing Diarrhea

Examples

Experimental program
Comparison scheme
Effect test

example 1

Selection of Probiotic Strains Intended for Preventing and / or Treating Diarrhea

Material and Methods

[0191]Probiotic Strains Tested.

[0192]The strains tested are as follows: a strain of Lactobacillus plantarum and the strain Bacillus subtilis CU1.

[0193]The cells of Bacillus subtilis CU1 (hereinafter CU1) are used in sporulated form and dry.

[0194]The cells of Lactobacillus plantarum (hereinafter LR) are used in a lyophilized, vegetative form.

[0195]Measurement of the Effect of a Probiotic Strain on Expression of CFTR and of NHE3.

[0196]Four mice were treated for 2 weeks, each with 109 CFU / day of CU1.

[0197]Four mice were treated for 2 weeks, each with 109 CFU / day of LR.

[0198]Four mice not treated with CU1 were used as the control.

[0199]The expression of CFTR and NHE3 was measured by immunohistochemistry on colonocytes obtained from mice treated with CU1 and from control mice, as described below.

[0200]Immediately after sacrifice of the mice, samples of the proximal colon (1 cm long) were wa...

example 2

Use of the Probiotic Strain CU1 in the Prevention and / or Treatment of Diarrhea

Material and Methods

[0204]Model of LPS-Induced Diarrhea

[0205]109 CFU / day / animal of the probiotic strain to be evaluated were administered via the intragastric route to male DBA / 2 mice for 14 days.

[0206]The mice in the control group (12 mice) were then treated with 200 μL of water administered intravenously and the mice in the test group (12 mice) received an administration of lipopolysaccharide (LPS) of Salmonella enteriditis (Sigma, Saint Quentin Fallavier, France) intravenously at a dose of 15 mg / kg, to induce diarrhea. The mice were placed in individual cages with the floor covered with a sheet of aluminum to allow collection of feces every 30 minutes for 120 minutes after injection of LPS. The fecal material collected was weighed and heated at 100° C. for 24 h and weighed again. The difference between the wet mass of the fecal material (before heating) and the dry mass corresponds to the excretion of w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
electrophoresisaaaaaaaaaa
mass spectrometryaaaaaaaaaa
Northern blotaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method of selecting or identifying probiotic strains capable of acting on the absorption of water in the colon, and use thereof as medicinal products in the treatment and / or prevention of diarrhea. The invention relates in particular to the strain of Bacillus subtilis CU1 for use in the treatment and / or prevention of diarrhea.

Description

RELATED PATENT APPLICATIONS[0001]The present patent application is filed pursuant to 35 U.S.C. §371 as a U.S. National Phase application of International Patent Application No. PCT / FR2013 / 053044, which was filed on Dec. 12, 2013, claiming the benefit of priority to French patent application number FR 12 61916 filed on Dec. 12, 2012. The International Application was published as WO 2014 / 091160 on Jun. 19, 2014. The content of each of the aforementioned patent applications is incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to the field of probiotic strains, in particular probiotic strains of Bacillus, for use in the treatment and / or prevention of diarrhea. The invention also relates to a method of selecting probiotic strains that have the particular feature of acting on the absorption of water in the colon.BACKGROUND OF THE INVENTION[0003]When functioning normally, the human colon absorbs about 99% of the water entering its lumen, w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/742C12R1/125G01N33/68
CPCA61K35/742C12R1/125A61K2035/115G01N2333/705G01N2500/10G01N33/6893G01N33/5044G01N33/56911A61P1/12C12N1/20A23L33/135C12R2001/125C12N1/205
Inventor KHUONG HUU, MARIEFIORAMONTI, JEANURDACI, MARIA
Owner INST NAT DE RECH POUR LAGRICULTURE LALIMENTATION & LENVIRONNEMENT