Nucleic acid amplification reaction apparatus and nucleic acid detection method

Abstract

A nucleic acid amplification reaction apparatus includes: a fitting section capable of fitting a nucleic acid amplification reaction vessel comprising a nucleic acid amplification reaction mixture; temperature adjustment sections which adjust the temperatures of first and second regions of the nucleic acid amplification reaction vessel, respectively; and a driving mechanism which switches the location of the first and second regions; the nucleic acid amplification reaction mixture includes first and second primer pairs, the reaction apparatus performs a thermal cycle by setting the temperatures of the first and second regions to a first temperature and a second temperature, respectively, and thereafter performs a thermal cycle by setting the temperatures of the first and second regions to a third temperature and a fourth temperature, respectively.

Description

BACKGROUND[0001]1. Technical Field[0002]The present invention relates to a nucleic acid amplification reaction apparatus and a nucleic acid detection method.[0003]2. Related Art[0004]In recent years, as a result of development of technologies utilizing genes, medical treatments utilizing genes such as gene diagnosis or gene therapy have been drawing attention. In addition, many methods using genes in determination of breed varieties or breed improvement have also been developed in agricultural and livestock industries. As technologies for utilizing genes, nucleic acid amplification technologies such as a PCR (Polymerase Chain Reaction) method have been widely used.[0005]In nucleic acid amplification technologies, as a method of simultaneously detecting a plurality of genes, multiplex PCR has been known (see, for example, JP-A-2010-207220).[0006]In multiplex PCR, however, in the case where a plurality of genes in one reaction mixture are detected, a plurality of primer pairs for the ...

AI Technical Summary

Benefits of technology

The invention provides a way to accurately and easily detect multiple genes in a single reaction system quickly and at a low cost. The method uses a single fluorescence detection system and reaction mixture, reducing the complexity of the operation. There is no need for dispensing, reducing the chance of errors. The method also avoids interference between primers and allows for quicker determination of amplification without the need for additional analysis.

Problems solved by technology

Accordingly, it was difficult to establish the reaction conditions.

Further, the inhibition of amplification reactions due to interference among respective primers caused by simultaneous amplification of a plurality of nucleic acids, which was a problem when a plurality of nucleic acids are amplified in one reaction mixture, does not occur.

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