Bacteroides CECT 7771 and the Use Thereof in the Prevention and Treatment of Excess Weight, Obesity and Metabolic and Immunological Alterations

Inactive Publication Date: 2017-07-06
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obesity is produced as a consequence of a positive and prolonged imbalance between intake and energy expenditure, which entails excessive gain in weight and body fat.
However, the excessive accumulation of fatty acids in the adipose tissue associated with obesity reduces insulin sensitivity, which promotes the accumulation of free fatty acids in the form of triglycerides in other organs and tissues (liver, muscle, heart, etc.), and causes alterations in leptin production or sensitivity, and an increase in the synthesis of proinflammatory cytokines, which in turn entails a greater risk of developing associated diseases (metabolic syndrome, hypertension, diabetes, cardiovascular diseases, etc.).
However, in obese subjects, the peripheral concentrations of this adipokine are abnormally high and produce resistance thereto.
Obesity is also characterised by alterations in the functions of various immune system cells, such as macrophages, dendritic cells and T cells, associated with reduced defences against pathogens and other antigens, and with a higher risk of infections and post-operative complications.
However, traditional treatments based on hypocaloric diets and increased physical activity are less effective at controlling obesity and, in general, lead to limited and temporary weight loss.
Neither has the use of pharmacological strategies been satisfactory,

Method used

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  • Bacteroides CECT 7771 and the Use Thereof in the Prevention and Treatment of Excess Weight, Obesity and Metabolic and Immunological Alterations
  • Bacteroides CECT 7771 and the Use Thereof in the Prevention and Treatment of Excess Weight, Obesity and Metabolic and Immunological Alterations
  • Bacteroides CECT 7771 and the Use Thereof in the Prevention and Treatment of Excess Weight, Obesity and Metabolic and Immunological Alterations

Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation and Identification of the B. uniformis CECT 7771 Strain.

[0132]We proceeded to the isolation of strains of the genus Bacteroides from faeces of healthy infants who had not been subjected to treatments with antibiotics for at least the month prior to sampling. The samples were kept at 4° C. and analysed within two hours after collection. Two grammes of each were diluted in 10 mM phosphate buffer containing a concentration of 130 mM NaCl (PBS) and homogenised in a Stomacher 400 Lab Blender (Seward Medical, London, UK) for 3 minutes and were diluted in peptone water. Aliquots of 0.1 ml of various decimal dilutions were inoculated onto Schaedler agar (Scharlau, Barcelona, Spain) supplemented with kanamycin (100 mg / L), vancomycin (7.5 mg / L) and vitamin K (0.5 mg / L), at 37° C. under anaerobic conditions. After incubation for 48 h at 37° C. under anaerobic conditions (AnaeroGen, Oxoid, UK), isolated colonies were selected and their morphology was confirmed under Gram staining. The...

example 2

Selection of the B. uniformis CECT 7771 Strain in Accordance With its Capability to Modulate, In Vitro, the Macrophage Response Related to Chronic Low-Grade Inflammation Associated With Obesity and Acccording to the Ability to Modify Lipid Accumulation and use of Glucose By Hepatocytes

2.1. Evaluation of the Effect of Bacterial Strains on Macrophages

[0136]Bacterial strains and culture conditions. The following strains of the genus Bacteroides were used: B. uniformis CAY1 (CECT 7771), B. uniformis CBD2, B. distasonis CAY3, B. fragilis SX3, B. fivegoldi SX2, B. dorei SS1, B. ovatus SV2, B. thetaiotaomicron SAC4 and B. caccae SV3. The strains were inoculated into 10 ml of Brain Heart broth (BH; Schartau Chemie S.A., Barcelona, Spain) containing 0.05% cysteine (BH), at 1% with 24 hours of culture were incubated for 22 h at 37° C. in anaerobiosis. (AnaeroGen; Oxoid, Basingstoke, UK). The cells were collected by centrifugation (6,000 g, 15 minutes), washed twice in PBS (10 mM sodium phosph...

example 3

Effect of Administration of the B. uniformis CECT 7771 Strain on Biometric and Bionchemical Parameters, Absorption of Lipids in the Intestine and Histology of Adipose Tissue and Liver

3.1. Animal Model of Obesity and Sampling.

[0145]Adult male C57BL-6 mice (6-8 weeks; Harlan Laboratorios) were used. The animals were kept under controlled temperature (23° C.), with a 12 hour light / dark cycle in an atmosphere with 40%-50% relative humidity.

[0146]Obesity was induced by feeding the mice a high-fat diet (HFD) which provided 60% of energy in the form of lipids (60 / Fat, Harlan Laboratorios) at the expense of a reduction in carbohydrates, for 7 weeks, while the non-obese mice were fed a conventional diet that provided 12.4% of energy as lipids. The mice had free access to water and diet. Weight was monitored weekly. The experiments were conducted in accordance with the rules of the Animal Ethics Committee.

[0147]The animals were randomly divided into four groups (n=6 / group): (1) controls that ...

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PUM

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Abstract

The invention relates to a strain of Bacteroides uniformes with registration number CECT 7771, and to the cellular constituents, metabolites and/or secreted molecules thereof. The invention also relates to a composition (nutritional or pharmaceutical) comprising at least one of the previous products. The invention further relates to the use of a strain of Bacteroides uniforms, preferably CECT 7771, or of the cellular constituents, metabolites and/or secreted molecules of said strain, or of a composition comprising same, for the prevention and/or treatment of alterations such as excess weight, obesity, adipocyte hypertrophy, hepatic steatosis or fatty liver, dyslipidemia, hyperglycemia, insulin resistance and diabetes, metabolic syndrome, hypertension, cardiovascular diseases, dysfunction of the immune system, reduced defences against infections, and imbalance in the composition of the intestinal microbiota.

Description

FIELD OF THE ART[0001]The present invention falls within the field of pharmaceuticals and food. Specifically, the present invention relates to the Bacteroides uniformis CECT 7771 strain, to its cellular components, metabolites and secreted molecules, and to compositions which, comprising at least one of the foregoing products, can also comprise other microorganisms or other compounds with biological activity. Likewise, the present invention also relates to the use of a B. uniformis strain or to the use of the CECT 7771 strain in the prevention and / or treatment of alterations such as excess weight, obesity, hepatic steatosis or fatty liver, dyslipidemia and, in particular, hypercholesterolemia and / or hypertriglyceridemia; hyperglycemia, insulin resistance and diabetes, preferably[0002]Type 2 diabetes mellitus and gestational diabetes; metabolic syndrome, hypertension, cardiovascular diseases, immune system dysfunction associated or not associated with these pathologies, preferably in...

Claims

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Application Information

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IPC IPC(8): A61K35/74A23L2/52A23L33/135A61K45/06
CPCA61K35/74A61K45/06A23V2002/00A23L33/135A23L2/52A61P1/16A61P3/00A61P3/04A61P3/06A61P3/10A61P9/00A61P9/12A61P31/00A61P37/00A61P37/04C12N1/20C12N1/205C12R2001/01A23C9/152A61K35/741
Inventor HERRANZ, YOLANDA SANZCANO, PAOLA GAUFFINSANTACRUZ, YOLANDA ARLETTEPEREZ, ANGELA MOYALLOPIS, MOISES LAPARRA
Owner CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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