Method for evaluation of grafts

a graft and evaluation technology, applied in the field of graft evaluation, can solve the problems of affecting so as to improve the effect of regenerative medicine, and facilitate the selection of graft samples

Inactive Publication Date: 2018-06-21
TERUMO KK
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach allows for the selection of graft samples with high transplantation potential, improving the effectiveness of regenerative medicine by ensuring high-quality grafts with enhanced tissue integration and cytokine production, thereby improving the accuracy and consistency of graft transplantation.

Problems solved by technology

However, the production of grafts for transplantation still involves technical difficulties and economical problems.
The problem is that grafts prepared and transplanted in the same way differ from one another in the ratio of cell “take” and the state of cell growth into tissues.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluation of grafts
  • Method for evaluation of grafts
  • Method for evaluation of grafts

Examples

Experimental program
Comparison scheme
Effect test

example 1

for Multinucleation

[0088]In order to examine the rate of multinucleation of sheet-shaped cell cultures of skeletal myoblasts, two kinds of sheet-shaped cell cultures (Sheet A and Sheet B) were prepared. The sheet-shaped cell cultures were examined for the rate of multinucleation according to the following protocol.

[0089]Each of the sheet-shaped cell cultures of skeletal myoblasts was subjected to an enzyme treatment, to be completely dissociated into mononuclear skeletal myoblasts. The skeletal myoblasts resulting from dissociation of Sheet A and Sheet B are designated respectively myo A and myo B. The dissociated cells were inoculated onto culture slides. The culture slides were placed in a CO2 incubator. The cells were cultured under the conditions of 37° C. and 5% CO2 for 14 to 22 hours.

[0090]After cultivation, the culture slides were removed from the CO2 incubator and observed under a microscope and imaged by a digital camera. The number of multinucleate cells was counted, and t...

example 2

n of Cytokine Yielding Capacity

[0091]Subsequently, the two kinds of sheet-shaped cell cultures of skeletal myoblasts, Sheet A and Sheet B, were examined for the capacity of yielding cytokine by using the following measurement kits. Quantikine Human VEGF (made by R&D Systems Inc.), Quantikine Human CXCL 12 / SDF-1α (made by R&D Systems Inc.), and RayBio Human HGF ELISA (RayBiotech, Inc.). Measurement was carried out according to the protocol specified by each kit.

[0092]The results are shown in FIGS. 1 and 2. The rate of multinucleation is around 16.8% for myo A and around 29.6% for myo B (see FIG. 1). As regards the cytokine yielding capacity, Sheet B is much higher than Sheet A for HGF, VEGF, and SDF-1 (see FIG. 2). These species of cytokine are said to be concerned with the regeneration of cardiac muscle. The foregoing results suggest that Sheet B, which is composed of skeletal myoblasts myo B having a high rate of multinucleation, is more competent for transplantation to the heart t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method and system for evaluating the competence for transplantation of a graft sample containing cells capable of multinucleation are provided. In one form, the method includes determining multinucleating ability as an indicator representing the capacity for multinucleation of the cells capable of multinucleation.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is continuation of prior U.S. application Ser. No. 13 / 766,833, filed Feb. 14, 2013, which is a continuation of International Application Number PCT / JP2011 / 068587 filed on Aug. 17, 2011, which claims priority of Japanese Application Number 2010-182142, filed Aug. 17, 2010, which are all hereby incorporated herein by reference in their entirety.[0002]The present invention relates to a method and system for evaluation of competence for transplantation of a graft.BACKGROUND OF THE INVENTION[0003]Despite recent innovative progresses in the therapy of heart diseases, no firm therapeutic system has been established yet for severe heart failure. A common method for treating heart failure is that of medical therapy by means of β-blockers or an ACE inhibitor. Heart failure too severe to be effectively cured by such therapy is treated by replacement therapy with an auxiliary artificial heart or by heart transplantation, i.e., surgic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12Q1/04G01N33/50A61K35/34C12N5/077
CPCA61K35/34G01N2800/245G01N33/5026C12N5/0658C12Q1/04
InventorSAKAMOTO, KENTA
OwnerTERUMO KK