Crystal Structures Comprising Elastin-Like Peptides
a crystal structure and peptide technology, applied in the direction of peptide/protein ingredients, crystal growth process, polycrystalline material growth, etc., can solve the problems of lack of appropriate alternatives, lack of regenerative capacity of mature enamel, and lack of functional highly organized materials for dental applications, etc., to achieve low bone density or osteoporosis
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example 1
Discussion and Results
Example 1
Discussion and Results
[0244]Membranes were fabricated as detailed above using three different ELPs comprising a statherin-derived peptide (SN), RGDS, or no bioactive segment (IK), where the only molecular difference is the presence of the bioactive sequence (Table 2). SN membranes have been shown to enhance mineralisation and therefore were used as the main experimental group in this study, while RGDS membranes were used for comparison, and IK membranes and borosilicate glass substrates coated with the different ELP molecules were used as controls. Surprisingly, upon incubation in a supersaturated solution rich in Ca2+, PO43−, and F− (10, 6, and 2 mM, respectively) at near physiological conditions (37° C. and pH 6.0), distinctive hierarchically mineralized structures (FIG. 30a-c) were observed on both sides of the SN and IK membranes (FIG. 30b) and not on the ELP-coated glass surfaces, which only showed flat platelet-like crystals (FIG. 35a-d). The res...
example 2
Micro-Fabricated ELP-Based Membranes
[0255]Method
[0256]Direct write laser lithograpy was used to fabricate a silicon chrome photomask. A (111)-oriented silicon wafer was coated with an 8 um thick layer of SU8-10 photoresist, soft baked (65 C for 2 minutes and 950 C for 5 minutes), and then exposed through the silicon chrome photomask (30 mWcm-2 for 3.3 seconds). It was subsequently post-exposure baked (65 C for 1 min and 950 C for 5 min) and developed using SU8 developer for 30 s. Topographies were transferred to polydimethylsiloxane (PDMS) using a standard soft lithography process. The PDMS prepolymer was poured on top of the patterned master, degassed under vacuum for 7 min, and then cured at 650 C for 2 hours. The resulting topographically patterned mold was subsequently used to create membranes with the different structural components using similar dropcasting method as mentioned for the smooth membranes.
[0257]Results
[0258]FIG. 14 shows the preferential nucleation and growth of f...
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