Method for extracting active ingredients

Inactive Publication Date: 2019-05-23
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0004]In view of the deficiencies of the conventional extraction techniques, the present invention provides a method for extracting an acti

Problems solved by technology

However, conventional methods are time-consuming, inefficient in extracti

Method used

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Examples

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Example

EXAMPLE

[0032]The present invention will be further illustrated in detail with the following specific examples, wherein the adopted measurement apparatuses and methods are respectively described as follows:

[0033]1. Measurement Apparatus:[0034](1) UV / VIS spectrophotometer: purchased from Jasco company, product no.: V-360; and[0035](2) pH meter: purchased from EUTECH company, product no.: pH510.

[0036]2. Measurement Method

[0037]2.1 Determination of Betalain Amount

[0038]The determination method comprises the following steps:[0039]1. Preparation of solutions:[0040](1) Na2HPO4 solution (200 mM): Taking 1.4196 g of Na2HPO4 and adding deionized water until the total volume is 100 mL.[0041](2) Citrate acid solution (200 mM): Taking 1.9213 g of citrate acid and adding deionized water until the total volume is 100 mL.[0042](3) Mcllvaine's buffer: Taking 53.6 mL of Na2HPO4 solution (200 mM) and adding 46.4 mL of citrate acid (200 mM), then adjusting the pH value to 5.2.[0043]2. Pre-treatment of ...

Example

Example 1

[0087]Shelled Chenopodium formosanum was subjected to a breaking process by a crusher for 30 seconds to obtain a powder of Chenopodium formosanum. The powder of Chenopodium formosanum was uniformly mixed with water (powder of Chenopodium formosanum: water 32 1:1-10, at a volume ratio). The mixture thus obtained was placed into a homogenizer. At a normal temperature or 85° C., a primary homogenization was conducted by using blades for coarse homogenization for 2 to 10 minutes. Then, after changing the blades to ones for fine homogenization, further homogenization was conducted for 2 to 10 minutes to obtain a crude extract (first extract).

[0088]The crude extract was subjected to a sieving process by a 400 mesh sieve to obtain a filtrate. Then, the filtrate was placed into a high pressure homogenizer and subjected to a high pressure homogenization. The high pressure homogenization was conducted at a temperature ranging from 25° C. to 85° C. and a pressure ranging from 200 bar ...

Example

Comparative Example 1

[0090]Shelled Chenopodium formosanum was grinded and the grinded Chenopodium formosanum was sieved (sieve: 10 mesh) to provide a powder of Chenopodium formosanum. The powder of Chenopodium formosanum was mixed with water (powder of Chenopodium formosanum: water=1:10, at a volume ratio), and then was extracted at 75° C. for 0.5 hour to provide a crude extract.

[0091]The crude extract was cooled to 25° C. and subjected to a centrifugation at a rotary speed of 5000 rpm for 10 minutes. The supematant thus obtained was filtered by a 400 mesh filter to obtain a filtrate. At 60° C., the filtrate was subjected to vacuum concentration to obtain a concentrated extract.

[0092]The amounts of active ingredients such as betalain, betaxanthin, total polyphenol and total flavonoid in the concentrated extract were determined by the aforesaid determination methods. The results are shown in Table 1.

TABLE 1Extraction results of active ingredients in Chenopodium formosanumHomogenizati...

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Abstract

A method for extracting active ingredient(s) from a raw material to be extracted is provided. The method comprises the following steps: (1) mixing a raw material to be extracted with an aqueous solvent to provide a mixture; (2) conducting a homogenization to the mixture to obtain a first extract with active ingredient(s); and (3) conducting a high pressure homogenization to the first extract to obtain a second extract with active ingredient(s), wherein the homogenization in step (2) is conducted at a temperature ranging from normal temperature to 100° C.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a method for extracting an active ingredient from a raw material to be extracted, and particularly relates to a method for extracting an active ingredient from a raw material to be extracted by using a high pressure homogenization.BACKGROUND OF THE INVENTION[0002]It is known that plants contain many active ingredients, which are beneficial for the human body, such as anthocyanidin in black tomatoes, serotonin in banana peels, and betalain, betaxanthin, polyphenol and flavonoid in Chenopodium formosanum, etc.[0003]It is known that the active ingredients in plants can be extracted by way of such as the dipping method, expeller process, solvent extraction method, ultrasonic extraction method or supercritical extraction method. However, conventional methods are time-consuming, inefficient in extraction, and unable to be used in mass-production, and thus should be improved.SUMMARY OF THE INVENTION[0004]In view of the deficienci...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07D211/82C07D309/10C07D311/76C07D209/44C07H17/02B01D11/02
CPCC07H1/08C07D211/82C07D309/10C07D311/76C07D209/44C07H17/02B01D11/0257B01D11/02B01D11/0292B01F33/82C07D209/16C07D211/92
Inventor LIN, YUNG-HSIANGLU, CHIEN-YU
Owner TCI CO LTD
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