Compositions and methods for treating and repairing tendons

a technology of tendons and compositions, applied in the field of compositions and methods for repairing tendons, can solve the problems of rupture or torn tendons, and the healing of tendons can take several years

Inactive Publication Date: 2019-12-26
REPLICEL LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The use of NBD cells leads to enhanced tendon repair and regeneration, reducing inflammation and improving healing times, making it suitable for treating a wide range of tendon injuries including tendinopathies and ruptures.

Problems solved by technology

Tendons can also be ruptured or torn, typically necessitating surgical intervention.
While there are a number of surgical methods that can be used to treat tendon injuries, even with such methods healing of the tendon can take several years, if they heal at all.

Method used

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  • Compositions and methods for treating and repairing tendons
  • Compositions and methods for treating and repairing tendons
  • Compositions and methods for treating and repairing tendons

Examples

Experimental program
Comparison scheme
Effect test

example 1

Tissue Sampling

[0042]A skin biopsy from the occipital area of the scalp is obtained from a subject as follows. Briefly, once an appropriate area of the scalp has been selected, it is shaved with hair clippers, ensuring some stubble remains. The biopsy area is then thoroughly disinfected and anaesthetized. Once anesthesia has taken effect, a 4-10 mm deep punch biopsy is gently removed from the biopsy site and the incision closed with sutures which can be removed 12-14 days later. The skin biopsy is then packaged under aseptic conditions into a pre-labelled biopsy tube containing biopsy transport medium, composed of DMEM / Hams F12 with antibiotics.

example 2

Isolation and Cultivation of NBDS Cells

[0043]A sterility test is performed on the medium in which the biopsy has been transported to ensure the sample is free from contamination, or alternatively, if the sample is contaminated to ensure that medium with antibiotics is subsequently utilized. The biopsy is then washed several times to remove the biopsy transportation medium and any debris to prepare the tissue for subsequent processing. Hair follicles are processed in Hams F10 by cutting away the skin epithelium with a sterile scalpel and “plucking” or dissecting the whole hair follicle unit from the surrounding dermal tissue using sterile forceps. The hair follicle is gripped with a forceps as close as possible to the skin surface and the follicle exposed by pulling up on the hair in the hair follicle unit. Follicles in the anagen phase (growing phase of the hair cycle, indicated by the visible outer root sheath, and DSC of the hair bulb) are selected for further processing.

[0044]NBD...

example 3

Preparation and Administration of NBDS Cells into a Tendon

[0047]Cells are prepared for use in two-chambered syringe. The first chamber contains approximately 20 million cells suspended in 1 ml of total volume. The second chamber contains 1.5 ml of autologous plasma from the patient (prepared separately before this procedure).

[0048]The two-chambered syringe is utilized to inject cells (under ultrasound guidance) into multiple locations of the tendon to be repaired.

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Abstract

The present invention relates to compositions and methods utilizing hair follicle derived Non-Bulbar Dermal Sheath cells for use in the treatment or prevention of the tendon injuries.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a divisional of U.S. application Ser. No. 14 / 767,577, filed Aug. 12, 2015, which was the National Stage of International Application No. PCT / US2014 / 016109, filed Feb. 12, 2014, which claims the benefit under 35 U.S.C. § 119(e) of U.S. Provisional Patent Application No. 61 / 763,908 filed Feb. 12, 2013, which applications are incorporated herein by reference in their entireties for all purposes.FIELD OF THE INVENTION[0002]The present invention relates to compositions and methods for repairing tendons, and more specifically, to compositions comprising non-bulbar dermal sheath cells for use in the treatment and repair of tendons, and for the prevention of tendon injuries.BACKGROUNDDescription of the Related Art[0003]Tendons are tough bands of fibrous connective tissue that usually connect muscle to bone. Examples of common tendons include the Achilles tendon, which connects the calf muscle to the heel bone, and the Patellar ...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61K35/36A61K45/06A61K38/30A61K38/20A61K38/19A61K38/18C12N5/0775C12N5/077C12N5/071
CPCA61K38/30C12N2501/113C12N5/0628A61K38/1833A61K38/204A61K38/1858A61K38/1808A61K38/193A61K38/1841A61K38/1825A61K38/18C12N5/0666A61K45/06A61K35/36C12N2501/905C12N5/066A61P21/00A61K2300/00
InventorHOFFMANN, ROLFMCELWEE, KEVIN JOHN
OwnerREPLICEL LIFE SCI