Structured illuminating microscopy and structured illuminating observation method
a structure illuminating and microscopy technology, applied in the direction of instruments, optical elements, fluorescence/phosphorescence, etc., can solve the problem of reducing the time taken to obtain all the required images, and achieve the effect of expanding the structure direction
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[0029]Hereinafter, a structured illuminating microscopy system will be described as an embodiment of the present invention.
[0030]FIG. 1 is a configuration diagram of a structured illuminating microscopy system of the present embodiment. As illustrated in FIG. 1, in the present system, there are disposed a coherent light source 1, a light intensity modulator 30, a collector lens 2, an ultrasonic wave spatial light modulator 3, a lens 4, a mask switching mechanism 5A, a lens 6, a field stop 5B, a lens 7, an excitation filter 8a, a dichroic mirror 8, a fluorescence filter 8b, a second objective lens 11, an imaging device (CCD camera or the like) 12, a controlling device 19, an image storing-calculating device (computer or the like) 13, an image displaying device 14, and an objective lens 9. Note that a reference numeral 10 in FIG. 1 denotes a specimen placed on a not-illustrated stage, and in this case, it is assumed that the specimen is previously fluorescent-stained.
[0031]The coheren...
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