100 results about "Laboratory assay" patented technology

The invention provides a testing method for the content of a coal seam methane sampled under a negative-pressure environment. The testing method comprises the following steps: sampling, namely carrying out in-situ measurement on the methane desorption amount; recording the final in-situ desorption amount V2; determining a methane desorption principle of a coal sample under the negative-pressure environment; carrying out lost methane amount calculation, namely calculating the loss amount V1 according to the measured methane desorption principle of the coal sample under the negative-pressure environment; carrying out residual methane amount laboratory determination, namely heating the coal sample in a sealed state and degassing in vacuum; testing the first-time degassing amount V3; crushing the coal sample by a ball grinding mill and degassing to determine the second-time degassing amount V4; carrying out gas component analysis in the degassing process; and determining the content X of the coal seam methane according to the V1, the V2, the V3 and the V4, and the weight m of the coal sample. The testing method for the content of the coal seam methane sampled under the negative-pressure environment is simple and feasible and particularly has important theoretical values and actual meanings on the desorption amount and the desorption principle of methane under the negative-pressure environment when negative-pressure pumping-out type spot sampling is carried out; the accuracy and the reliability of testing data can be greatly improved and theoretical data are provided for the coal mine safety production.

The invention discloses an on-site sealed sampling device for coal bed gas. The device comprises a hollow long rod piece, wherein the outer circumference of the long rod piece is provided with a spiral convex edge; a coal storage cabin, a closed cabin, a transmission cabin and a power and control cabin are arranged in the long rod piece from front to back respectively; the closed cabin is provided with a coal inlet; a reciprocating push and pull mechanism is arranged in the transmission cabin; a micro power speed reducing motor for providing power for the reciprocating push and pull mechanism is arranged in the power and control cabin; the front end of the reciprocating push and pull mechanism is in transmission connection with a piston sliding sleeve device for opening and closing the coal inlet; and the front end of the coal storage cabin is provided with a gas resolution hole. The device is mainly used for a sampling link before the process of measuring the content of the gas in a laboratory, has high practical value and is easy to popularize and apply, the on-site sealed acquisition of a coal sample and the gas is realized, underground resolution is not needed, loss amount is not required to be calculated in the process of measuring the content of the gas, and reliable basic parameters are provided for related fields of coal mine gas prevention and control and the like.

The invention relates to a method for predicting three-dimensional abnormal pore pressure based on rock physical seismic information. The method comprises the steps that the lithologic stratum matrix bulk modulus is calculated by applying parameters of the rock physical longitudinal wave velocity, transverse wave velocity and density, rock core geological analysis is additionally conducted so as to calculate the dry pore spatial rigidity, the pore pressure coefficient is obtained accordingly, and the single-well stratum abnormal pore pressure is calculated; and secondly, a three-dimensional data cube of the longitudinal wave impedance, the transverse wave impedance, Poisson ratio and the density is obtained by applying a three-dimensional seismic reflection data cube and three-dimensional prestack elastic wave impedance retrieval method, and the three-dimensional stratum abnormal pore pressure is predicted by reference to the single-well stratum abnormal pore pressure calculation method and an obtained empirical formula. According to the method for predicting the three-dimensional abnormal pore pressure based on the rock physical seismic information, rock physical parameters obtained indirectly through logging and seismic geophysical methods are applied to prediction of the abnormal stratum pore pressure, and the practice is easier compared with direct measuring of rock physical parameters in a laboratory, more scientific compared with subjective parameter presetting, and finer compared with calculation separately through post-stack seismic velocity.

The invention relates to a greenhouse gas collection system and a greenhouse gas emission analysis method. A collection device comprises an overwater gas collection device and a gas storage device connected with the overwater gas collection device. The overwater gas collection device comprises a gas collection tank, a gas collection fan, a gas collection pipe, a battery box, a handle and a foaming plate. The gas storage device comprises a gas collection bag and a three-way valve. By utilizing the greenhouse gas device, gas samples are collected at the nearshore positions of the two shore sides of a river and water-gas interfaces of a channel, a fish pond, a reservoir and the like, the collected samples are taken back to a laboratory, and the concentration of greenhouse gases of CO2, CH4 and N2O3 in the gas samples is measured. The collection device is simple and reasonable in structural design, low in cost and easy and convenient to operate and use, has the good stability and sealing performance. The analysis method is simple in operation steps, convenient to implement, small in experiment error, high in accuracy, and capable of effectively solving the problem that river greenhouse gases are difficult to collect.

The invention discloses a vehicle evaporative emission operation loss VOCs emission factor measurement method. Based on serial applications of multiple modes, the operation loss emission can be indirectly and quantitatively judged. The method comprises steps of light-duty gasoline vehicle and heavy-duty diesel vehicle exhaust emission laboratory measurement, light-duty gasoline vehicle hot dipping emission VOCs emission factor laboratory measurement, actual road vehicle VOCs comprehensive emission factor tunnel measurement, VOCs species chemical composition analysis and model analysis. Based on the light-duty gasoline vehicle exhaust emission and hot dipping emission in a laboratory airtight cabin or a drum, a single process characteristic source spectrum is built. The model analysis comprises chemical mass balance model analysis and comprehensive emission factor deduction. Operation loss emission factors of a vehicle group in an actual road condition are finally built. The technology makes up for the existing lacked vehicle VOCs operation loss emission testing condition, emission errors caused by inconsistency between the experimental condition and the actual condition when the vehicle VOCs operation loss is tested in the laboratory in the past can be solved, and the vehicle VOCs operation loss emission factors can be accurately acquired.

The invention provides a method and device for determining TOC content. The method comprises the following steps: establishing a TOC optimally-estimated mineral rock physical model by utilizing data of laboratory-measured X-ray diffraction data, organic carbon density and logging density curve and the like; obtaining accurate TOC distribution on a well through an optimization iteration method; establishing a TOC quantity version by utilizing actually-measured TOC data and an equivalent medium model; determining TOC sensitivity elastic parameters; and finally, carrying out prestack elastic parameter inversion on earthquake reflection gather obtained through OVT region processing. The method and device for determining the TOC content realize the purpose of quantitatively forecasting space distribution of the TOC content by utilizing well-seism integration through statistical fitting, and solve the problem of not high TOC content forecasting result; and through using the laboratory-measured data and the rock physical model effectively, the estimated result is not only matched with the laboratory-measured value at the places of rock samples, but also can predicate the TOC content at the places without sampling points accurately.

The invention relates to a device for determining a soil breathing rate in a laboratory and a method for determining the soil breathing rate, and in particular relates to a device for determining the breathing rate of the soil and a method for determining the soil breathing rate by utilizing the device, and the device and the method are used for solving the problems that the existing device for determining the soil breathing rate that time is wasted in operation and the standard error is large. The device consists of a culture device and an absorption device, wherein the culture device consists of a culture bottle and a rubber plug, the absorption device consists of an absorption bottle and a rubber plug, the culture bottle is connected with the absorption bottle by virtue of an inner ground branch pipe and an outer ground branch pipe, and the outer ground branch pipe is inserted into the inner ground branch pipe to form the closed connection. The application method comprises the following steps: I, preparing a solution; II, preparing the device; III, culturing; IV, determining the soil breathing rate; V, re-culturing; VI, re-determining the soil breathing rate, and repeating the steps V and VI for 17 to 56 times to complete the determination. The device and the method are applied to the field of the laboratory analysis.

The invention relates to a method for calculating gas loss according to a relation between an accumulated desoprtion value and the time. A large quantity of experiments show that the relational equation between the accumulated desorption value in the initial stage after pressure of coal cuttings is released is V=A(t / T)<n>, but the power exponent n is not a constant, is related to the gas pressure and is changed along with the time; accurate determination of the power exponent n of the accumulated desorption value is a key for calculation of the gas loss. In order to accurately calculate the gas loss, the method comprises the steps of 1, firstly evaluating the gas pressure of a coal seam through on-site measurement; 2, recording the observation time t, the accumulated desorption value Vt with the time t, the total desorption time T and the loss time t0 during the on-site measurement of the gas content; 3, measuring the value of n in a lab under a condition of the gas pressure of the coal seam to be measured: obtaining a unitary regression relational equation between lnVt and ln[(t+t0)n-t0n] through Excel by a least square method, wherein an intercept is ln(A / Tn), and substituting the n value and the T value to obtain an A value; and 4, substituting the A value, the T value the n value into V=A(t0 / T)<n> to obtain the gas loss V.

A kind of multifunctional autosampler, it is characterized in that, mainly comprises 101-sample tray (horizontal rotor); 102-sample vial; 103-sample tray driving motor; 104-driving motor; 105-leading screw; 106-cleaning liquid 107‑base; 201‑sampling needle; 202‑washing needle; 203‑slider; 204‑spring; valve; 301-port of multiport valve and 201 injection needle; 302‑port of multiport valve and 202 cleaning needle; 303‑test water in line with laboratory use; 304‑cleaning solution bucket (bottle) (such as acid solution or Alkaline solution); 305-Reagent barrel (bottle); 306-Waste liquid barrel (bottle); 307-Mixed reactor; 308-Standard solution barrel (bottle). It can be used for automatic sampling in the determination of seawater, high-chloride and high-salt wastewater, surface water, groundwater, drinking water, domestic sewage, and industrial wastewater; it can be used for automatic sampling in laboratory determination, online determination, and emergency portable on-site determination.

The invention discloses a tester for researching a compression-permeation relationship of garbage soil, which aims at filling up the blank of an instrument for researching a compression-permeation relationship of garbage soil in landfill at present. According to the tester, one side of a permeation column is connected with a plurality of water outlet joints and the other side of the permeation column is connected with a plurality of piezometric tubes which are arranged equidistantly; an adjustable bottom plate is connected with a water level adjusting rod through pins; a water storage tank is communicated with the permeation column through a water inlet pipe and is connected with a plurality of water outlet joints; a gland is connected with an upper crossbeam through a suspension rod; a lower crossbeam, a first balancing weight and a second balancing weight are hinged with a lever; both a hub of a worm wheel and a worm are borne in a support through bearings; the worm wheel is meshed with the worm; one end of the worm extends out of the support to be fixed with a hand wheel; the worm wheel is connected with a screw rod; the bottom of the screw rod is hinged with the lever. According to the tester, the mechanical laboratory measurement on the permeation property and the compression property of garbage soil can be performed and the research gap of the compression and permeation of garbage soil from existing instruments is filled.

The invention relates to a sampling device and a sampling method for accurate determination of coal seam gas content. The device comprises a hollow drill rod and is characterized in that: the hollow drill rod is used for fixing a high energy power rod by a connection board which is placed in connection grooves and by connection groove screwed holes, and is used for fixing a sampling drill bit by the connection board which is placed in the connection grooves and by the connection groove screwed holes. The device and the combined sampling method realize instantaneous positional sampling with punching at any angles, considering of loss amount is not needed, the sampled coal sample is an original coal sample, and the sample is directly sealed in the sampling drill bit; the drill bit is taken out and directly sent to a laboratory for determination, so that sampling without gas loss amount is basically realized, and determination accuracy of the gas content is improved.

The invention relates to a star sensor external field conversion reference measurement system and a star sensor external field conversion reference measurement method. The system comprises a star sensor 1, an astronomical north theodolite 2, a theodolite A3, a theodolite B4, a global positioning system (GPS) antenna 5, a GPS receiver 6, a gas pressure temperature sensor 7 and a control computer 8;the method uses the system provided by the invention; the method applies the fixed stars as a measurement reference to determine the conversion relationship between a measurement coordinate system ofthe star sensor and a reference prism coordinate system. The system and the method avoid the estimation error caused by the parameter coupling, of parameters such as a principal point in a calibration process of the star sensor in the traditional laboratory test method. The measurement accuracy of the conversion reference is effectively improved. The method is widely applicable to the determination of external lead-out references for various types of star sensors.

The invention discloses a method and a device for quickly determining a coal seam gas parameter for an underground coal mine, and belongs to methods and devices for determining coal seam gas parameters. The method comprises: establishing a database about a gas parameter and a desorption curve by utilizing a one-to-one correspondence, determined by a lab, between the coal seam gas parameter and the desorption curve; matching underground field desorption data with the desorption curve in the database to acquire the coal seam gas parameter thereof. A coal seam gas parameter quick determinator of the determination device is provided with a flow meter, a single-chip computer, a display, a data storage device, a lithium battery power supply, and an intrinsic safety control circuit, and is connected with a coal sample tank through a gas guide pipe to determine values of coal seam gas pressure, gas content and drillings gas desorption indexes (K1 and K2). The advantages are as follows. The method can determine multiple coal seam gas parameters simultaneously, the needed time is short, the workload is small, the manufacturing cost is low, the operation is simple, the determination result is high in reliability, a judgment index is provided for outburst coal seam danger evaluation, and the method is of great significance for the development of an outburst prevention technology for a workface.

The invention relates to a device for measuring leakage of a water supply pipeline and a measuring method using the device, in particular to a device for measuring leakage of a water supply pipeline in a laboratory and a measuring method using the device. The device for measuring leakage of the water supply pipeline in the laboratory and the measuring method using the device are used for solving the problems that in the prior art, a method for detecting the relation between the leakage amount and the pressure of a water supply pipeline cannot achieve the purpose of measuring the leakage amount of leaking openings with different shapes, or when a nighttime minimum flow method is used for measurement by collecting regional pipe network data, the method is complex and high in time consumption, and regional normal water supply is influenced. The device is composed of a controllable valve, a tail end pressure gauge, a vent valve, a casing pipe, a front-end pressure gauge, the water supply pipeline, a hoop, a volume flowmeter, a water containing device, a surge bank, a water pump, a water tank and a programmable logic controller. The measuring method comprises the steps of adjusting the water pressure and measuring the leakage amount. The device for measuring leakage of the water supply pipeline in the laboratory and the measuring method using the device are implemented.

The invention discloses a method for measuring the ammonium ion content of coal ash and belongs to the technical field of desulfurization with ammonia. According to the forming process of a coal ash sample desulfurized with ammonia, the variety of the ammonia salt and the intrinstic chemical characteristic of the coal ash sample, the aspects of sample treatment, determination mechanism, operating steps, result calculation and the like are subjected to creative invention, the existing formaldehyde method is improved, and compared with the titrimetric method after distillation, the method has the characteristics of few experiment preparation content, simple operation, low time consumption, small experiment error and the like; and according to the method for measuring the ammonium ion content of the coal ash, the ammonia ion content of the coal ash generated by the desulfurization technology with ammonia is analyzed accurately, a scientific basis is provided for reasonable utilization of the coal ash desulfurized with ammonia, and the absolute difference of the parallel measuring result is not more than 0.06 percent. The absolute difference of different laboratory measuring results is not more than 0.12 percent.

The invention relates to a pressure-maintaining airtight coal sample collecting device and method. Compared with an existing coal mine coal sample collecting device, coal sample collecting is conducted in a closed environment, the escape of gas in a coal sample is avoided, and therefore the gas content of the coal sample is obtained by calculating the sum of the coal sample on-site gas desorptionamount and the coal sample residual gas desorption amount measured in a laboratory; the coal sample collecting device is simple in operation, high in applicability, simple and convenient to maintain,safe and efficient; the coal sample is collected during drilling of a drilling stem, it can be ensured that the coal sample is in an airtight state in the collecting process, the gas loss in the coalsample is avoided, and the coal mine downhole gas content measuring accuracy is effectively improved.

A fluorescence analysis reagent kit for detecting homocysteine in blood is disclosed. It uses a novel internal standard thiohydroxy acetic acid for quantity control and the chromatographic peaks of said internal standard and homocysteine can be clearly separated from each other. Its advantages are less specimen, high correctness, high speed, and low cost.

The invention provides a near infrared determination method for potato reducing sugar. The method comprises the following steps: a step of establishing a determination model; a step of collecting a sample; a step of carrying out laboratory determination: determining the content of reducing sugar of potato samples of different varieties by using laboratory chemical analysis; a near infrared analysis step: carrying out vacuum freeze drying on the potato samples with different varieties to obtain freeze-dried potato samples; grinding the freeze-dried potato samples into powder and putting the powder into a sample tank to be subjected to near infrared spectrum scanning to obtain a near infrared spectrum; a step of establishing a prediction model: establishing the prediction model according to the near infrared spectrum of the samples and a chemical near infrared spectrum and establishing a mathematic model between the measured value of the potato reducing sugar and the near infrared spectrum; and a determination step: substituting the near infrared spectrum of a sample to be detected into the mathematic model established in the step of establishing the prediction model to obtain the content value of the potato reducing sugar of the sample to be detected.

An underground soft coal seam simulating and copying method comprises the steps as follows: a raw coal sample of an underground soft coal seam is acquired and subjected to moisturizing treatment, andthe apparent density and moisture of the sample are determined in a laboratory; the coal sample of the soft seam is added to a small soft seam pressing device to be pressed under different pressures,and average crustal stress of the coal seam is applied; after the coal sample is taken out, the apparent density of the coal sample in a stress-free state is determined and compared with the actual apparent density of the soft seam, and the forming pressure stress of the soft seam can be obtained; a coal sample of the soft seam is acquired underground and screened to be in the preset size and thenthe moisture of the coal sample is determined; quantitative water is added, stirring is performed, pressurized forming is performed in a large soft seam pressing forming device, and the average crustal stress of the coal seam is applied; vacuum pumping is performed, the coal seam is filled with gas under different pressures, and the simulated and copied coal seam of the soft seam with different outburst hazards is obtained. Favorable conditions are provided for detecting sensitivity of the coal seam to outburst prediction indexes and outburst critical values. The method is simple, convenient,easy to operate, reliable and accurate.

Systems and methods are used to generate a protocol for an assay. At least one performance characteristic parameter of an assay and at least one standardized protocol for each assay of a plurality of assays and assay types are stored. A performance characteristic parameter selection and an assay selection are received from a client device of a laboratory. One or more performance characteristic parameters and a standardized protocol are retrieved from the database device. The client device is sent the one or more performance characteristic parameters and one or more study variable values. One or more amendments to the one or more performance characteristic parameters and one or more study variable values are received from the client device. A protocol for the assay is generated based on the one or more amendments.

The invention relates to the technical field of photovoltaics, and discloses a photovoltaic annual generating capacity calculation method based on machine learning, which comprises the following steps: S1, applying a time sequence correlation model to predict irradiance: a1, collecting monthly actual measurement meteorological data of all meteorological stations in a region where a power station is located in history of 10 years, averaging the data, and fitting the data by utilizing an ARp-order autoregressive process; a2, calculating parameter items through simulation, and predicting the monthly average radiation value and temperature of the next year; a3, calculating an illumination intensity distribution curve of the photovoltaic power station in the past year, namely the ratio of illumination time to annual illumination time under each illumination intensity; s2, predicting the generating capacity by utilizing a sample plate assembly method: b1, taking ten sample plate assemblies from different matrixes of a power station, and determining an IV curve and a temperature coefficient of the sample plate assemblies in a laboratory; the photovoltaic annual generating capacity calculation method based on machine learning can solve the problem that simulation carried out by existing mainstream photovoltaic power station simulation software is rough at present.

The invention discloses a device for determining field moisture capacity of soil with different particle sizes indoors. The device comprises a soil sample glass container (1), wherein one side of the surface of an iron stand (3) is provided with a column in the position near the edge, and the column is provided with a big iron ring (5); the soil sample glass container (1) is vertically arranged in the big iron ring (5); a big beaker (4) is arranged on the surface of the iron stand (3), and the upper opening of the big beaker (4) is faced with the bottom of the soil sample glass container (1); and the bottom of the soil sample glass container (1) is provided with a plastic bottom cover (2), thereby solving the problem of laboratory determination of field moisture capacity of soil with different particle sizes.

The invention provides a measuring device for chloride ions and total organic carbon in seawater, high-chloride and high-salt waste water, surface water, underground water, drinking water, domestic sewage and industrial waste water. Mainly include, 1-quantitative sampling device; 2-acidification reactor; 3-oxidation reactor; 4-condenser; 5-gas chromatography column; 6-thermal conductivity detector (TCD); 7-halogen remover; 8-Non-dispersive infrared detector (NDIR); thermal conductivity detector (TCD) channel, non-dispersive infrared detector (NDIR) channel; can be used for laboratory measurement, online measurement, emergency portable on-site measurement.

The present invention comprises rugged, inexpensive, reliable, and sensitive laboratory assays of antibody-based viral neutralization activity and antibody-based viral adherence inhibition activity. The assays use inactivated, fluorescently-labeled virus, allowing the tests to be performed without extensive safety precautions. The interaction of the labeled virus with target cells is monitored using flow cytometric methods. A preferred embodiment uses simple and inexpensive flow cytometry methodologies and equipment, such as bead array readers used as simplified flow cytometers. The assays are rapid, taking no longer than a few hours and are readily conducted by a trained technician. The assays are sensitive because they use labeled viruses at low concentrations and determine neutralizing and blocking capacity of sera and antibody at low concentrations. The methods are appropriate for high-throughput screening of large panels of samples.

The invention provides an apparatus for measuring chlorine ion and total organic carbon in sea water, high-chlorine and -salt wastewater, surface water, underground water, drinking water, domestic sewage and industrial wastewater, wherein the apparatus mainly includes a sample quantitative sampling device, a potentiometric titrator, a horizontal rotor, a sample dish, a sample cup (sample tube), an automatic sample injector, a multi-position valve, an oxidization reactor, a gas-liquid separator, a membrane filter and a detector, and a data processing and display apparatus. The apparatus can be used for measuring the chlorine ion and the total organic carbon in water for measurement in laboratories, on-line measurement and emergent portable in-site measurement.

The invention discloses a method for using waste ion exchange resin ball to test soil available phosphorus, which comprises (1) regenerating waste ion exchange resin ball, (2) using lab assay method or using field in-situ assay method, (3) taking and washing the resin ball via some deionized water, eluting two times via alcaine solution at 0.5mol / L density, collecting all eluent, (4) using general molybdenum-antimony anti-colorimetry to test the phosphorus density in the eluent. The inventive method has low cost, simple operation and accurate test result.

The invention relates to a content detection method for lysine of a compound ketoacid tablet, which comprises the following steps: preparing an o-phthalaldehyde reaction liquid (OPA reagent) with the concentration of 10 mg / ml, wherein 2-3 percent (v / v) of 3-thiohydracrylic acid is contained; afterwards, mixing lysine and the reaction liquid at ratio of 1:1(v / v) in a borate buffer system with a pH valve of 10.2 plus or minus 0.1 through an automatic pre-column derivation method, and then carrying out ambient temperature reaction; and finally, adopting a high performance liquid chromatography to measure the content of the lysine at 338 nm. The method provided by the invention can effectively solve the problem that bimodality of the lysine occurs in the current OPA pre-column derivation method, improves the sample introduction precision of the lysine in the detection through the automatic pre-column derivation method, greatly shortens the detection time, and is particularly suitable for detecting the content of the lysine of amino acid samples in a laboratory.

The present invention refers to new derivatives of the penta-1,4-dien-3-ones, as well as their processes of preparation. These compounds present strong antitumoral activity and promising antiparasitic action, behaving as almost atoxic by laboratory assays and also by hystopathologic studies. The present invention refers also to a pharmaceutical composition including the referred compounds, method of treatment for cancer and parasitic diseases.

The invention relates to a particle organic carbon and total organic carbon determinator and a determination method. The determinator and the method are characterized in that a carrier gas generator is orderly communicated with a gas purifier and a flow controller through pipelines, the flow controller is respectively communicated with a high-temperature oxidation reactor and a low-temperature decomposition reactor through pipelines, and the other end of the high-temperature oxidation reactor and the other end of the low-temperature decomposition reactor are respectively communicated with a condensation dehydrator through pipelines; the condensation dehydrator, an ion trap, a gas filter and a conductivity gauge are orderly communicated through pipelines; and the conductivity gauge is connected with a data processor through a cable. By the determinator and the method provided by the invention, the total carbon, the inorganic carbon, the particle organic carbon, the total organic carbon and so on in the solid, particle, liquid and suspension samples can be respectively determined, so that determination of the particle organic carbon in soil, sludge, organic chemical residues and so on and the total organic carbon in surface water, domestic sewage and production waste drainage is realized; and the determinator and the method are wide in measurement range and convenient to use, and can perform laboratory determination and vehicle-mounted mobile determination, thereby being worth spreading.

The invention provides a whole-course acquisition method of dissolved inorganic carbon content and carbon isotope value of an environmental water body. By using a saturated ammonium dihydrogen phosphate solution (blended when used), the problems of inconvenience during using process of phosphoric acid and the long reaction time are solved. In the laboratory, high-purity helium (99.999%) is used toclean the residual CO2 in the 12mL headspace bottle. A saturated ammonium dihydrogen phosphate solution can be injected in the laboratory in advance, or the saturated ammonium dihydrogen phosphate solution can be injected in the field. Meanwhile, the environmental water body can be firstly injected in the field, and saturated ammonium dihydrogen phosphate is then injected. Thereby, the operationis flexible and convenient. The quality control of the DIC content and isotope determination of the environmental water sample from the field sample treatment to the laboratory measurement process isrealized, and the true representation of the DIC content and the isotope data of the field water body can be ensured to the greatest extent. The method of the invention has great prominent effect on the research of the influence of global carbon cycle and human activity on water body pollution.