The invention belongs to the technical field of medical instruments, and particularly relates to a puncture needle suite assembly and a using method thereof.The puncture needle suite assembly comprises a puncture needle suite assembly body and a hose, the puncture needle suite assembly body is composed of a puncture sheath, a blood vessel dilator, an obturator, a miniature guide wire and a puncture needle, and a third hollow pipe is fixedly installed at one end of the hose; a first hollow tube is fixedly installed on the inner wall of the top end of the puncture needle, the inner wall of the first hollow tube is detachably connected with a third hollow tube through a fixing structure, and a sealing structure is arranged at one end of the puncture needle. Compared with the prior art, the tail end of the puncture needle is prevented from being injected into a contrast agent, so that the phenomenon that the puncture needle moves is avoided, and the situation that blood cannot be seen after blood return is punctured is avoided, so that great convenience is brought to medical staff.