The invention relates to a multichannel microfluidic device used for diagnosis of
cholera and containing a highly-hydrophobic
chip substrate, belonging to the field of
analytical testing. According tothe invention, a series of problems are generated in manufacturing of a substrate of a
microfluidic chip used for diagnosis of
cholera from cheap and easily-processed
polydimethylsiloxane, i.e., PDMS. The main points of a technical scheme of the invention are as follows: the material of the substrate is PDMS with a nascent surface; the interiors of the channels of a
chip are filled with doped micron-sized
titanium dioxide particles, wherein the super-hydrophilic properties of the surface of the
titanium dioxide particles can be induced out through preset visible-
light irradiation; an assemblyof gaps and channels among particles of the
titanium dioxide particle filling material compensates and surpasses original highly-hydrophobic channels, so constant and super-hydrophilic modification effects are achieved; meanwhile, through a manner of
coupling operation combining
micropower ultrasonic-wave driving and
micropump driving, a test specimen liquid flow is guided to flow towards the terminal direction along the channels of the
chip.