Culture medium for thermo-acid resistant bacterium
A technology of culture medium and acid-resistant bacteria, which is applied in the field of culture medium to achieve the effects of cost saving, accelerated growth and shortened inspection cycle
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Embodiment 1
[0012] This embodiment is made up of the raw material of following proportioning:
[0013] Peptone 5g Glucose 5g Yeast extract 5g
[0014] Dipotassium hydrogen phosphate 4g Soluble starch 5g Agar powder 20g
[0015] 3.08% manganese sulfate solution 0.5mL distilled water 1000mL.
[0016] The preparation method of the present embodiment is: the above-mentioned Add peptone, glucose, yeast extract, dipotassium hydrogen phosphate, soluble starch, agar powder and distilled water into the Erlenmeyer flask, mix well, heat to dissolve, adjust the pH to 4.5, then add 0.5mL of 3.08% manganese sulfate solution into the Erlenmeyer flask, Mix evenly, and sterilize in an autoclave at 110°C for 20 minutes to obtain the finished product.
Embodiment 2
[0018] This embodiment is made up of the raw material of following proportioning:
[0019] Peptone 6g Glucose 6g Yeast Extract 6g
[0020] Dipotassium hydrogen phosphate 3g Soluble starch 3g Agar powder 20g
[0021] 3.08% manganese sulfate solution 0.5mL distilled water 1000mL.
[0022] The preparation method of the present embodiment is: the preparation method of the present embodiment is: the above-mentioned Add peptone, glucose, yeast extract, dipotassium hydrogen phosphate, soluble starch, agar powder and distilled water into the Erlenmeyer flask, mix well, heat to dissolve, adjust the pH to 4.5, then add 0.5mL of 3.08% manganese sulfate solution into the Erlenmeyer flask, Mix evenly, and sterilize in an autoclave at 110°C for 20 minutes to obtain the finished product.
Embodiment 3
[0024] This embodiment is made up of the raw material of following proportioning:
[0025] Peptone 4g Glucose 6g Yeast Extract 4g
[0026] Dipotassium hydrogen phosphate 5g Soluble starch 5g Agar powder 20g
[0027] 3.08% manganese sulfate solution 0.5mL distilled water 1000mL.
[0028] The preparation method of the present embodiment is: the preparation method of the present embodiment is: the above-mentioned Add peptone, glucose, yeast extract, dipotassium hydrogen phosphate, soluble starch, agar powder and distilled water into the Erlenmeyer flask, mix well, heat to dissolve, adjust the pH to 4.5, then add 0.5mL of 3.08% manganese sulfate solution into the Erlenmeyer flask, Mix evenly, and sterilize in an autoclave at 110°C for 20 minutes to obtain the finished product.
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