Method for inhibiting biogenic sulfide generation
A technology for sulfide production and biology, applied in chemical instruments and methods, botanical equipment and methods, biological water/sewage treatment, etc., and can solve problems such as impractical use
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Embodiment 1
[0048] Inhibition of Sulfide Production by Desulfovibrio Desulfovibrio G100A in Response to Anthracene Diol
[0049] Thaw 1 mL of the frozen culture of Desulfovibrio desulfuricans G100A (Desulfovibrio desulfuricans G100A), inject it into a test tube containing 10 mL of reducing-modified BTZ-3 medium covered by the septum, and incubate at 30 °C for 3 days (all during the experiment The transfer process, substance addition and sampling were performed in an anaerobic chamber at room temperature. The cultivation was performed outside this chamber.). 5 mL of this culture was transferred to a 60-mL septum-covered serum bottle containing 50 ml of reduced BTZ-3 medium and incubated overnight at 30°C. 3 ml of this culture was transferred to 50 ml of modified Postgate's B medium in a 60-mL serum bottle covered with a septum seal. A total of 6 cultures were thus prepared. The above four samples were then treated with two concentrations (100 ppm and 500 ppm) of the anthracene diol sal...
Embodiment 2
[0065] Anthracene diol inhibits sulfide production by Desulfovibrio desulfovibrio G100A
[0066] Thaw 1 mL of the frozen Desulfovibrio desulfovibrio G100A culture, inject it into a septum-covered test tube containing 10 mL of reduced-modified BTZ-3 medium, and incubate at 30°C for 3 days (all transfer procedures, material Addition and sampling were performed in an anaerobic chamber at room temperature. Incubation was performed outside the chamber.). 5 mL of this culture was transferred to a 60-mL septum-covered serum bottle containing 50 ml of reduced BTZ-3 medium and incubated overnight at 30°C. 3 ml of this culture was transferred to 50 ml of modified Postgate's B medium in a 60-mL serum bottle covered with a septum seal. A total of 6 cultures were thus prepared. The above four samples were then treated with two concentrations (140 ppm and 700 ppm) of the anthracene diol salt solution in duplicate by injecting the material into a septum vial with a microliter syringe and...
Embodiment 3
[0082] Two different anthracene diol salt solutions in inhibiting the sulfidation produced by Desulfovibrio desulfovibrio G100A comparison of things
[0083] Thaw 1 mL of the frozen Desulfovibrio desulfovibrio G100A culture, inject it into a septum-covered test tube containing 10 mL of reduced-modified BTZ-3 medium, and incubate at 30°C for 3 days (all transfer procedures, material Addition and sampling were performed in an anaerobic chamber at room temperature. Incubation was performed outside the chamber.). 5 mL of this culture was transferred to a 60-mL septum-covered serum bottle containing 50 ml of reduced BTZ-3 medium and incubated overnight at 30°C. 3 ml of this culture was transferred to 50 ml of modified Postgate's B medium in a 60-mL serum bottle covered with a septum seal. A total of 14 cultures were thus prepared. The substance was then injected into the analyte by using a microliter syringe with the following three concentrations (5, 10 and 50 mg / l equivalen...
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