S protein of SARS virus, its vaccine and method for screening medicament using it

A technology of SARS virus and protein, applied in the field of characteristic sites of SARS virus, can solve the problem that the decisive site of SARS virus toxicity and transmission ability is not reported, etc.

Inactive Publication Date: 2008-01-09
SOUTH RES CENT NAT HUMAN GENE GROUP +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the decisive sites for the virulence and transmissibility of the SARS virus have not been reported so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Detection of SARS mutant S protein

[0059] Collect virus isolation and culture samples from multiple SARS patients in different regions from the early stage of the epidemic to the late stage (January 5, 2003 to April 7, 2003), isolate viral nucleic acid, and then obtain complementary DNA (cDNA ), or directly obtain cDNA from biological samples, such as sputum, feces and tissues and organs by reverse transcription reaction, and perform full sequence determination to obtain the S protein sequence.

[0060] The results are shown in Table 1.

[0061] Table 1 lists the sites and nucleotide changes of SARS virus S protein mutations.

[0062] TOR2

S1nt

AAA

S1 codon

S1aa

SNVs

aa

Isolation with SNVs*

21715

224

75

aCg

Thr

wxya

Arg

S30, S08, C52, C53

21721

230

77

gG

Gly

gA

Asp

numerous cases

22207

716

239

Ca

Ser

tT

Leu

S30, S08, C...

Embodiment 2

[0067] Detection of SARS mutant S protein II

[0068] The virus isolation and culture samples from three SARS patients in Guangzhou in the early stage of the epidemic (January-February, 2004) and the SARS virus samples from civet cats were collected. By the same method as in Example 1, the complete sequence of the isolated SARS virus was determined, and the S protein sequence was obtained therefrom. The results are shown in Table 2.

[0069] Table 2 lists the sites and nucleotide changes of early SARS virus S protein mutations.

[0070] TOR2

S1nt

AAA

S1 codon

S1aa

SNVs

aa

Isolation with SNVs*

22570

1079

360

TT

Phe

TC

Ser

GZ03-01, GZ03-02, GZ03-03

22927-

22928

1436

479

AAt

Asn

AGA or

aAA

Arg or

Lys

civet virus

22930

1439

480

wxya

Asp

gG

Gly

GZ03-01, GZ03-02, GZ03-03

23316-

23317

1825

...

Embodiment 3

[0077] SARS detection kit

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PUM

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Abstract

The present invention discloses a S protein of SARS virus, which has SEQ ID NO.2 aminophenol sequence, and has mutation of (a) 778D to Y; (b) 77D to G; (c) 244T to I; (d) 1182K to Q; (e) 360F to S; (f) 479N to R or K; (g) 480D to G; (h) 609A to L. the invention also discloses nucleic acid encoding the S protein and SARS virus containing the mutations. The invention further discloses medicament for screening the SARS and its vaccine of the S protein aiming at SARS virus.

Description

technical field [0001] The present invention relates to the fields of virology, epidemiology and molecular genetics, and more specifically relates to the characteristic loci of SARS virus and its application. Background technique [0002] In 2003, my country and many countries in the world were hit by a large-scale atypical pneumonia (Atypical pneumonias). occur. The World Health Organization (WHO) quickly established a collaborative research and monitoring network consisting of 13 laboratories in 10 countries around the world after the outbreak. On April 16, WHO officially confirmed that the pathogen of SARS was a new type of coronavirus, which is a single-stranded RNA virus. [0003] There have been more than 8,000 cases of atypical pneumonia in the world, and the death rate in some diseased areas has reached 15%, which has brought great panic to the people. Carrying out research on the etiology, prevention, diagnosis and treatment of SARS coronavirus in a timely manner,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/165A61K39/215A61P31/14A61P11/00C12Q1/68C12Q1/04
Inventor 赵国屏林锦炎宋怀东郑焕英王升跃何剑峰任双喜鄢心革李亦学万卓越孔祥银许锐恒郝沛吴新伟顾伯伟侯金林陈竺闵军缪有刚徐军傅刚陆家海张树义王小宁涂长春王鸣
Owner SOUTH RES CENT NAT HUMAN GENE GROUP
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