Biological deodorization and purification agent, and its use
A technology for biological deodorization and purifying agent, which is applied in the fields of biochemical equipment and methods, deodorization, and microorganisms, and can solve the problems of odorous gas resources and waste.
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Embodiment 1
[0078] Embodiment 1, the cultivation of Candida utilis (Candida utilis) fermented liquid
[0079] 1) Plate culture rejuvenation: the slanted seeds of Candida utilis were inoculated by streaking on the plate medium of Candida utilis, and cultured at 27°C for 20-24 hours to rejuvenate Candida utilis , and form a single colony; the Candida utilis plate medium is a mixture prepared in the following proportions: soybean peptone 0.5g, glucose 1.0g, yeast extract powder 0.3g, malt extract 0.5g, agar powder 2g, pH=5.8, 100ml of water, sterilized at 121°C for 15 minutes, set aside;
[0080] 2) Making shake flask seeds: Pick a single colony from the Candida utilis culture plate cultivated in step 1), inoculate it into the liquid culture medium of the Erlenmeyer flask, and cultivate it at 30°C for about 30 hours to obtain a shake flask of Candida utilis Seed liquid; the culture liquid is a mixed liquid prepared according to the following proportions: 0.5g soybean peptone, 1.0g glucose, ...
Embodiment 2
[0084] Embodiment 2, the cultivation of lactobacillus (Lactobacillus sp.) fermented liquid
[0085] Take Lactobacillus plantarum as an example
[0086] 1) Plate culture rejuvenation: the slanted seeds of Lactobacillus plantarum were streaked and inoculated on the plate medium of Lactobacillus plantarum by streak inoculation, and cultured at 35° C. for 36 hours to rejuvenate Lactobacillus plantarum and form a single colony; The Lactobacillus plantarum plate medium is a mixture prepared in the following proportions: soybean peptone 0.7g, glucose 1.5g, yeast extract powder 0.5g, potassium dihydrogen phosphate 0.4g, beef extract 1.0g, triammonium citrate 2.0g, Tween-80 1.0ml, sodium acetate 0.4g, agar powder 2.0g, water 100ml, pH=5.6, sterilized at 121°C for 15 minutes, set aside;
[0087] 2) Make shake flask seeds: pick a single colony from the Lactobacillus plantarum colonies cultivated in step 1), inoculate it into the Lactobacillus plantarum liquid medium, and cultivate it on...
Embodiment 3
[0091] Embodiment 3, the cultivation of Streptococcus lactis acidi (Streptococcus lactis acidi)
[0092] The same method as in Example 2 was used to cultivate the fermentation broth of Streptococcus lactis.
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