Secretory expression for human insulin gene in methyl alcohol yeast

A technology of human insulin and methanol yeast, applied in the field of genetic engineering, can solve the problem of low expression level, achieve the effects of increasing production, simple process, and shortening working hours
CN100460508CInactive Publication Date: 2009-02-11马延高 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
马延高
Publication Date
2009-02-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention was involved in expression of human insulin gene, especially for the expression of human insulin gene in Pichia Pastoris GS115 / pPICM#101 whose storage number wais CCTCC NO.M204071. It contained B and A strand synthesis, construction of express plasmid and engineering strain, transform of host cell, filter of transformant and over expression engineering strain, etc. The method left out the difficult procedure, reduced the process steps and shortened time, so the technique was simple. It overexpressed 10-100 folds than the others. It provided one new simple method for human insulin commercial process.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and it relates to the secretory expression of a human insulin gene, in particular to the expression of the human insulin gene in methanol yeast (Pichia Pastoris) GS115 / pPICM # 101. The secretion expression method in the deposit number CCTCCNO.M204071. Background technique

[0002] Insulin is the only natural hormone secreted by B cells that lowers blood sugar. Its main function is to regulate the content of glucose in the blood and transport glucose to certain target cells in the body, and participate in the metabolism of the three major substances and energy in the body. Too high or too low blood sugar in the human body is a disease. No matter what the cause is, the decrease in insulin secretion, which is not needed for the regulation of blood sugar in the body, will cause the patient to develop insulin-dependent diabetes mellitus (IDDM). Insulin is necessary to treat these patients. Human...

Claims

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