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Method for constructing HPLC standard fingerprint pattern of semen cuscutae medicinal materials and quality identification

A standard fingerprint and fingerprint technology are applied in the field of constructing the HPLC standard fingerprint of Cuscuta chinensis medicinal materials and identifying the quality of medicinal materials, which can solve problems such as unreported, and achieve the effects of enhanced identification, high precision and easy to grasp.

Inactive Publication Date: 2009-05-27
MASSON GROUP
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, some scholars have studied the method of detecting three flavonoids in Cuscuta by HPLC, but there is no report on the method of detecting multiple components of Cuscuta at one time and establishing a fingerprint by reversed-phase high-performance liquid chromatography

Method used

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  • Method for constructing HPLC standard fingerprint pattern of semen cuscutae medicinal materials and quality identification

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Construction of Dodder HPLC Standard Fingerprint

[0028] 1. Instruments and reagents

[0029] 1.1. Instrument

[0030] Agilent 1100 liquid chromatograph, DAD detector; Shanghai Brain SB5200 ultrasonic cleaner.

[0031] 1.2. Test drug

[0032] Methanol, analytically pure; acetic acid, analytically pure; acetonitrile, chromatographically pure, produced by Dima Company; ultrapure water, Wahaha purified water; 10 batches of dodder medicinal materials produced in Inner Mongolia.

[0033] The above-mentioned dodder medicinal materials have been tested to meet the corresponding national standard requirements in the Pharmacopoeia.

[0034] 2. Chromatographic conditions

[0035] The detection wavelength is 350nm; Dima C18 column (200×4.6mm, 5μm); the column temperature is 30°C; the flow rate is 1ml / min; the mobile phase is acetonitrile and 1% acetic acid solution, gradient elution, and the elution ratio is:

[0036] T(min) CH3CN(%) 1%CH3COOH(%)

[0037] 0 5 95

[0038...

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Abstract

The invention relates to construction dodder medicines HPLC standard fingerprint pattern and identification medicine quality method, used for the dodder medicine quality identification, and it discloses the powder of the trial materials ultrasonically extracting 60mins in the 20ml80% methanol solution, and filtering to obtain the trial materials solution; HPLC conditions: chromatogram column Dumas C18 column (200X4.6 mm, 5mum), column temperature 30deg.C; mobile phase acetonitrile-1% acetic acid solution, gradient elution, flow rate 1ml / min; detection wavelength 350nm. Taking 10 groups Inner Mongolia dodder medicines to determine, to find common characterized peak, with software plausible to establish a standard fingerprint pattern, there are totally six common characterized peaks, T1 to T6 peaks, retention time being 31.8min, 44.3min, 46.2min, 46.7 min, 50.1min, 68.4min; and then, compare the fingerprint pattern determined by the trial medicine and the standard fingerprint pattern, the medicine with similarity more than 0.900 is applied in the product.

Description

technical field [0001] The invention relates to a method for constructing an HPLC standard fingerprint of the dodder medicinal material and a method for identifying the quality of the medicinal material by using the standard fingerprint. Background technique [0002] Cuscuta medicinal material is planted in many provinces of my country, and its quality is uneven due to different varieties, origins, and harvesting periods, so it is difficult to guarantee the quality, efficacy, and stability of its finished products. The key part of the quality control of dodder in the current national pharmacopoeia standard is microscopic identification. These are obviously not enough for authenticity identification and quality evaluation of this medicinal material. At present, there are many studies on the chemical constituents in Cuscuta, and it is reported that it contains flavonoids, sterols and other types of constituents. However, the active ingredient of dodder is still not very clea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/26G01N30/86
Inventor 侯峰刘芳华文俊莫启武程朝晖王宝东王鸿昶刘汉槎
Owner MASSON GROUP
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