Preparation of fungi extract and application thereof in resisting HIV
A technology for extracts and fungi, applied to the field of preparation of endophytic fungi extracts, can solve the problems of untreated, easy to produce drug resistance, destruction of vegetation and human living environment, etc.
Inactive Publication Date: 2009-07-08
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology
However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to develop drug resistance; on the other hand, chemotherapy drugs have strong toxic and side effects, and most of these drugs are expensive, and many AIDS patients in economically backward countries and regions cannot afford medical expenses, so they cannot receive timely and effective treatment
[0004] Searching for anti-HIV drugs with high efficiency and low toxicity from traditional Chinese medicine has always been a very active field in the development of new drugs at home and abroad, but the large collection of plants may lead to the depletion of plant resources, resulting in the destruction of vegetation and human living environment
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[0019] 1. Fermentation of fungi
[0020] After activation of Epulorhiza sp. fungi from slant test tubes preserved at low temperature, they were transferred to ф90mm plates containing PDA medium, cultured at a constant temperature of 25°C for 12 days, and holes were punched at the edges of the colonies to form Bacterial flakes, and put into liquid culture medium with small fragments for fermentation culture;
[0021] The medium composition of fungal liquid culture is: glucose 20 g / L, KH 2 PO 4 3 g / L, MgSO 4 1.5 g / L; natural product is wheat bran 30 g / L (boiled juice), culture medium pH5.5. 500 mL Erlenmeyer bottle culture medium 200 mL, sterilized at 121 °C for 30 minutes, inoculated with Epulorhiza sp. ). Culture conditions: shaking speed 110 rpm; 25°C dark cultivation for 10 days to harvest.
[0022] 2. Obtaining of Fungal Fermentation Culture
[0023] After the fermentation and cultivation of fungi of the genus Epulorhiza (Epulorhiza sp.), filter through a 100-mesh nyl...
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An application of an extract of a plant endophytic fungus fermentation product in anti-human immunodeficiency virus (HIV). The fungus used is isolated from the root of the orchid medicinal plant Dendrobium, and is a fungus of the genus Epulorhiza EF1 (Epulorhiza sp. ); EF1 is cultured by liquid fermentation to prepare the extract of the fermentation product. The fermentation process of this bacterium is simple, the growth period is short, and it can be produced on a large scale; the extract of the fermentation product of this bacterium is easy to obtain, and has significant anti-HIV-1 activity, and its action site for HIV-1 infection is after reverse transcription Before the expression of HIV RNA, it has the function of regulating the expression of HIV-1 gene; drug combination experiments show that the combined use of the fermentation product of this bacteria and the anti-AIDS drug AZT shows an additive anti-HIV-1 effect. The extract of the fermentation product of the bacterium is of great value in the development of new drugs for the treatment of AIDS, and is expected to become a new drug for the treatment of AIDS.
Description
technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, it relates to a preparation technology of an endophytic fungus extract which has anti-human immunodeficiency virus (HIV-1) activity and can regulate HIV-1 gene expression. technical background [0002] Acquired Immunodeficiency Syndrome, also known as AIDS (AIDS), was first reported by the US Centers for Disease Control in 1981. In the mid-1980s, AIDS had developed into a global epidemic, and its pathogen was identified as Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV). Over the past two decades, research in the field of AIDS pathology and treatment has achieved certain results. In recent years, high-efficiency antiviral treatment has significantly reduced the morbidity and mortality of AIDS. However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to produce drug resistance; o...
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IPC IPC(8): C12N1/14A61K36/06C12P1/02A61P31/18
Inventor 郭顺星蒋岩相子春陈晓梅王春兰肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI