Medical application of fruit extraction of miracle fruit
A technology of Sacred Fruit and extract, which is applied in the field of medical application of Sacred Fruit extract, and can solve problems such as being unable to effectively inhibit
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Embodiment 1
[0036] Example 1: Preliminary test on the regulation of blood sugar by Mira holy fruit mixture
[0037] 1.1 Source of experimental animals
[0038]Male Wistar rats were obtained from the Animal Center of National Cheng Kung University School of Medicine, Taiwan, China, and were all over 8 weeks old and weighed between 200 and 250 grams. The rats used are all raised in the center, the air conditioner maintains the room temperature at 25±1°C, and the light time and dark time are 12 hours respectively. Rats had free access to food and water.
[0039] 1.2 Induction of insulin-dependent (type 1) diabetic rats
[0040] According to the commonly used method, after the 8-week-old rats of the aforementioned strain were fasted, they were anesthetized by intraperitoneal injection of 30 mg / kg pentobarbital, and 65 mg / kg of streptozotocin (STZ) was injected from the femoral vein; STZ was dissolved in Sodium citrate (10mmol / L) pH4.5 saline. One week after the injection, blood was collec...
Embodiment 2
[0064] Embodiment 2: Control test to normal and diabetic rats
[0065] 2.1 Regulating test of Mira holy fruit mixture on normal rats
[0066] Rats that had been fasted overnight were orally given different doses of Mira saccharomyces mixture, respectively 40 mg / kg, 60 mg / kg, and 80 mg / kg, and then anesthetized with pentobarbital to draw blood. see image 3 And as shown in Table 3, after 60 minutes, it was found that the 40mg / kg dose of Mira holy fruit mixture could produce a hypoglycemic effect in normal rats, and the blood sugar level dropped from the original 108.4±2.0mg / dL to 98.7±2.9mg / dL. dL, the hypoglycemic percentage reached 9.0±2.3%, and the hypoglycemic effect appeared in a dose-dependent manner. In addition, the hypoglycemic effect of the Mira holy fruit mixture also increased with time, and its effect would reach the maximum after 90 minutes of force-feeding.
[0067] Table 3. The hypoglycemic effect of force-feeding Mila ginseng fruit mixture on normal rats
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